Overview

  • Product name
    Anti-Ube2G2 antibody [EPR9248(2)]
    See all Ube2G2 primary antibodies
  • Description
    Rabbit monoclonal [EPR9248(2)] to Ube2G2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ube2G2 aa 1-100. The exact sequence is proprietary.
    Database link: P60604

  • Positive control
    • Mouse testis, rat testis, Jurkat, HeLa and K562 cell lysates. HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function
      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt
    • Database links
      see all
    • Alternative names
      • Human ubiquitin conjugating enzyme G29 antibody
      • UB2G2_HUMAN antibody
      • UBC 7 antibody
      • UBC7 antibody
      • UBC7 homolog yeast antibody
      • UBE2G2 antibody
      • Ubiquitin carrier protein G2 antibody
      • Ubiquitin conjugating enzyme 7 antibody
      • Ubiquitin conjugating enzyme E2 G2 antibody
      • Ubiquitin conjugating enzyme E2G 2 (homologous to yeast UBC7) antibody
      • Ubiquitin conjugating enzyme E2G 2 (UBC7 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2G 2 (UBC7 homolog, yeast) antibody
      • Ubiquitin conjugating enzyme E2G 2 antibody
      • Ubiquitin conjugating enzyme G2 antibody
      • Ubiquitin protein ligase G2 antibody
      • Ubiquitin-conjugating enzyme E2 G2 antibody
      • Ubiquitin-protein ligase G2 antibody
      see all

    Images

    • Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      All lanes : Anti-Ube2G2 antibody [EPR9248(2)] (ab174296) at 1/1000 dilution

      Lane 1 : Mouse testis lysate
      Lane 2 : Rat testis lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 19 kDa

    • Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

      Western blot analysis on immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab174296 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

      Immunofluorescent analysis of HeLa cells labeling Ube2G2 with ab174296 at 1/100 dilution (red). DAPI nuclear staining (blue).

    References

    This product has been referenced in:
    • Wang T  et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). Read more (PubMed: 28985237) »

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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