Recombinant
RabMAb

Recombinant Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

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Overview

  • Product name

    Anti-Ube2G2 antibody [EPR9248(2)]
    See all Ube2G2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9248(2)] to Ube2G2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ube2G2 aa 1-100. The exact sequence is proprietary.
    Database link: P60604

  • Positive control

    • Mouse testis, rat testis, Jurkat, HeLa and K562 cell lysates. HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab174296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Human ubiquitin conjugating enzyme G29 antibody
      • UB2G2_HUMAN antibody
      • UBC 7 antibody
      • UBC7 antibody
      • UBC7 homolog yeast antibody
      • UBE2G2 antibody
      • Ubiquitin carrier protein G2 antibody
      • Ubiquitin conjugating enzyme 7 antibody
      • Ubiquitin conjugating enzyme E2 G2 antibody
      • Ubiquitin conjugating enzyme E2G 2 (homologous to yeast UBC7) antibody
      • Ubiquitin conjugating enzyme E2G 2 (UBC7 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2G 2 (UBC7 homolog, yeast) antibody
      • Ubiquitin conjugating enzyme E2G 2 antibody
      • Ubiquitin conjugating enzyme G2 antibody
      • Ubiquitin protein ligase G2 antibody
      • Ubiquitin-conjugating enzyme E2 G2 antibody
      • Ubiquitin-protein ligase G2 antibody
      see all

    Images

    • Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      All lanes : Anti-Ube2G2 antibody [EPR9248(2)] (ab174296) at 1/1000 dilution

      Lane 1 : Mouse testis lysate
      Lane 2 : Rat testis lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 19 kDa

    • Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

      Western blot analysis on immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab174296 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

      Immunofluorescent analysis of HeLa cells labeling Ube2G2 with ab174296 at 1/100 dilution (red). DAPI nuclear staining (blue).

    References

    Publishing research using ab174296? Please let us know so that we can cite the reference in this datasheet.

    ab174296 has been referenced in 2 publications.

    • Wang T  et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). PubMed: 28985237
    • van de Weijer ML  et al. Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. J Cell Sci 130:2883-2892 (2017). PubMed: 28743740

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