Recombinant Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
![Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)](https://www.abcam.com/ps/products/174/ab174296/Images/ab174296-199513-anti-ube2g2-antibody-epr92482-western-blot.jpg "Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9248(2)] to Ube2G2
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Ube2G2 antibody [EPR9248(2)]
See all Ube2G2 primary antibodies -
Description
Rabbit monoclonal [EPR9248(2)] to Ube2G2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Ube2G2 aa 1-100. The exact sequence is proprietary.
Database link: P60604 -
Positive control
- Mouse testis, rat testis, Jurkat, HeLa and K562 cell lysates. HeLa cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9248(2) -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
|
|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
|
|
| IP |
1/10 - 1/100.
|
| Notes |
|---|
|
ICC/IF
1/100 - 1/250. |
|
WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa. |
|
IP
1/10 - 1/100. |
Target
-
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD). -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
-
Database links
- Entrez Gene: 7327 Human
- Entrez Gene: 22213 Mouse
- Entrez Gene: 294331 Rat
- Omim: 603124 Human
- SwissProt: P60604 Human
- SwissProt: P60605 Mouse
- Unigene: 529420 Human
- Unigene: 701398 Human
see all -
Alternative names
- Human ubiquitin conjugating enzyme G29 antibody
- UB2G2_HUMAN antibody
- UBC 7 antibody
see all
Images
-
Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)All lanes : Anti-Ube2G2 antibody [EPR9248(2)] (ab174296) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 19 kDa -
![Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)](https://www.abcam.com/ps/products/174/ab174296/Images/ab174296-199517-anti-ube2g2-antibody-epr92482-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)Western blot analysis on immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab174296 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
-
![Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)](https://www.abcam.com/ps/products/174/ab174296/Images/ab174296-199516-anti-ube2g2-antibody-epr92482-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)Immunofluorescent analysis of HeLa cells labeling Ube2G2 with ab174296 at 1/100 dilution (red). DAPI nuclear staining (blue).
-
![Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)](https://www.abcam.com/ps/products/174/ab174296/Images/ab174296-4-benefits-of-recombinant-antibodies.png)
Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab174296 has been referenced in 2 publications.
- Wang T et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). PubMed: 28985237
- van de Weijer ML et al. Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. J Cell Sci 130:2883-2892 (2017). PubMed: 28743740