RecombinantRabMAb

Recombinant Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

Submit a review Submit a question References (2)
Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
  • Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
  • Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9248(2)] to Ube2G2
  • Suitable for: ICC/IF, WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ube2G2 antibody [EPR9248(2)]
    See all Ube2G2 primary antibodies

  • Description

    Rabbit monoclonal [EPR9248(2)] to Ube2G2

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Ube2G2 aa 1-100. The exact sequence is proprietary.
    Database link: P60604

  • Positive control

    • Mouse testis, rat testis, Jurkat, HeLa and K562 cell lysates. HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab174296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
IP
1/10 - 1/100.
Notes
ICC/IF
1/100 - 1/250.
WB
1/1000 - 1/5000. Predicted molecular weight: 19 kDa.
IP
1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • Human ubiquitin conjugating enzyme G29 antibody
      • UB2G2_HUMAN antibody
      • UBC 7 antibody
      • UBC7 antibody
      • UBC7 homolog yeast antibody
      • UBE2G2 antibody
      • Ubiquitin carrier protein G2 antibody
      • Ubiquitin conjugating enzyme 7 antibody
      • Ubiquitin conjugating enzyme E2 G2 antibody
      • Ubiquitin conjugating enzyme E2G 2 (homologous to yeast UBC7) antibody
      • Ubiquitin conjugating enzyme E2G 2 (UBC7 homolog yeast) antibody
      • Ubiquitin conjugating enzyme E2G 2 (UBC7 homolog, yeast) antibody
      • Ubiquitin conjugating enzyme E2G 2 antibody
      • Ubiquitin conjugating enzyme G2 antibody
      • Ubiquitin protein ligase G2 antibody
      • Ubiquitin-conjugating enzyme E2 G2 antibody
      • Ubiquitin-protein ligase G2 antibody
      see all

    Images

    • Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      All lanes : Anti-Ube2G2 antibody [EPR9248(2)] (ab174296) at 1/1000 dilution

      Lane 1 : Mouse testis lysate
      Lane 2 : Rat testis lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate
      Lane 5 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 19 kDa

    • Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

      Western blot analysis on immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab174296 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.

    • Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

      Immunofluorescent analysis of HeLa cells labeling Ube2G2 with ab174296 at 1/100 dilution (red). DAPI nuclear staining (blue).

    • Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
      Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)

    References (2)

    Publishing research using ab174296? Please let us know so that we can cite the reference in this datasheet.

    ab174296 has been referenced in 2 publications.

    • Wang T  et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). PubMed: 28985237
    • van de Weijer ML  et al. Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. J Cell Sci 130:2883-2892 (2017). PubMed: 28743740

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