Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)
- Datasheet
- References (2)
- Protocols
Overview
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Product nameAnti-Ube2G2 antibody [EPR9248(2)]
See all Ube2G2 primary antibodies -
DescriptionRabbit monoclonal [EPR9248(2)] to Ube2G2
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Host speciesRabbit
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Tested applicationsSuitable for: ICC/IF, WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide within Human Ube2G2 aa 1-100. The exact sequence is proprietary.
Database link: P60604 -
Positive control
- Mouse testis, rat testis, Jurkat, HeLa and K562 cell lysates. HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR9248(2)
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IsotypeIgG
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Research areas
Associated products
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab174296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/1000 - 1/5000. Predicted molecular weight: 19 kDa. | |
| IP | 1/10 - 1/100. |
Target
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FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Involved in endoplasmic reticulum-associated degradation (ERAD).
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
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Database links
- Entrez Gene: 7327 Human
- Entrez Gene: 22213 Mouse
- Entrez Gene: 294331 Rat
- Omim: 603124 Human
- SwissProt: P60604 Human
- SwissProt: P60605 Mouse
- Unigene: 529420 Human
- Unigene: 701398 Human
see all -
Alternative names
- Human ubiquitin conjugating enzyme G29 antibody
- UB2G2_HUMAN antibody
- UBC 7 antibody
see all
Images
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![Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)](https://www.abcam.com/ps/products/174/ab174296/Images/ab174296-199513-174296.JPG)
Western blot - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)All lanes : Anti-Ube2G2 antibody [EPR9248(2)] (ab174296) at 1/1000 dilution
Lane 1 : Mouse testis lysate
Lane 2 : Rat testis lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 19 kDa -
![Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)](https://www.abcam.com/ps/products/174/ab174296/Images/ab174296-199517-1742963.JPG)
Immunoprecipitation - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)Western blot analysis on immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab174296 at 1/10 dilution. Detection utilized HRP-conjugated anti-rabbit IgG which preferentially detects the non-reduced form of rabbit IgG.
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![Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)](https://www.abcam.com/ps/products/174/ab174296/Images/ab174296-199516-1742962.JPG)
Immunocytochemistry/ Immunofluorescence - Anti-Ube2G2 antibody [EPR9248(2)] (ab174296)Immunofluorescent analysis of HeLa cells labeling Ube2G2 with ab174296 at 1/100 dilution (red). DAPI nuclear staining (blue).
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wang T et al. Enterovirus 71 protease 2Apro and 3Cpro differentially inhibit the cellular endoplasmic reticulum-associated degradation (ERAD) pathway via distinct mechanisms, and enterovirus 71 hijacks ERAD component p97 to promote its replication. PLoS Pathog 13:e1006674 (2017). Read more (PubMed: 28985237) »
- van de Weijer ML et al. Multiple E2 ubiquitin-conjugating enzymes regulate human cytomegalovirus US2-mediated immunoreceptor downregulation. J Cell Sci 130:2883-2892 (2017). Read more (PubMed: 28743740) »
