Overview

  • Product name

    Anti-UBE2I / UBC9 antibody
    See all UBE2I / UBC9 primary antibodies
  • Description

    Rabbit polyclonal to UBE2I / UBC9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human UBE2I/ UBC9 (internal sequence). The exact sequence is proprietary.
    Database link: P63279

  • Positive control

    • WB: HEK-293T, A431, HeLa and HepG2 whole cell extracts; Mouse testis tissue extract. IP: HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab227918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/100 - 1/500.
WB 1/500 - 1/3000. Predicted molecular weight: 18 kDa.

Target

  • Function

    Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
  • Tissue specificity

    Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
  • Pathway

    Protein modification; protein sumoylation.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Cellular localization

    Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
  • Information by UniProt
  • Database links

  • Alternative names

    • C358B7.1 antibody
    • p18 antibody
    • SUMO 1 protein ligase antibody
    • SUMO conjugating enzyme UBC9 antibody
    • SUMO-conjugating enzyme UBC9 antibody
    • SUMO-protein ligase antibody
    • SUMO1 protein ligase antibody
    • UBC9 antibody
    • UBC9_HUMAN antibody
    • UBCE9 antibody
    • Ube2i antibody
    • Ubiquitin carrier protein 9 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein I antibody
    • Ubiquitin conjugating enzyme 9 antibody
    • Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody
    • Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody
    • Ubiquitin conjugating enzyme UbcE2A antibody
    • Ubiquitin like protein SUMO 1 conjugating enzyme antibody
    • Ubiquitin protein ligase E2I antibody
    • Ubiquitin-conjugating enzyme E2 I antibody
    • Ubiquitin-protein ligase I antibody
    see all

Images

  • UBE2I / UBC9 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract using 5 μg of ab227918. Western blot was performed from the immunoprecipitate using ab227918. 

    Lane 1: HEK-293T whole cell extract.

    Lane 2: Control in HEK-293T whole cell extract.

    Lane 3: ab227918 in HEK-293T whole cell extract.

  • Anti-UBE2I / UBC9 antibody (ab227918) at 1/1600 dilution + Mouse testis extract at 50 µg

    Predicted band size: 18 kDa



    15% SDS-PAGE gel.

  • All lanes : Anti-UBE2I / UBC9 antibody (ab227918) at 1/1000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 18 kDa



    15% SDS-PAGE gel.

References

ab227918 has not yet been referenced specifically in any publications.

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