• Product name
    Anti-UBE2I / UBC9 antibody
    See all UBE2I / UBC9 primary antibodies
  • Description
    Goat polyclonal to UBE2I / UBC9
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Hamster, Dog, Xenopus laevis
  • Immunogen

    Synthetic peptide:


    , corresponding to N terminal amino acids 2-14 of Human Ube2I.

  • Positive control
    • Human Kidney, Human Lung and Human Testis lysates
  • General notes
    Product is very sensitive to freeze/thawing therefore this should be avoided. Store at 4C for short term use and -20C for long term storage in small aliquots.



Our Abpromise guarantee covers the use of ab21193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 18 kDa). Transfer onto PVDF membrane and block in 3 % milk. Incubate primary antibody with membrane at room temperature.
ELISA 1/64000.


  • Function
    Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation.
  • Tissue specificity
    Expressed in heart, skeletal muscle, pancreas, kidney, liver, lung, placenta and brain. Also expressed in testis and thymus.
  • Pathway
    Protein modification; protein sumoylation.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Cellular localization
    Nucleus. Cytoplasm. Mainly nuclear. In spermatocytes, localizes in synaptonemal complexes. Recruited by BCL11A into the nuclear body.
  • Information by UniProt
  • Database links
  • Alternative names
    • C358B7.1 antibody
    • p18 antibody
    • SUMO 1 protein ligase antibody
    • SUMO conjugating enzyme UBC9 antibody
    • SUMO-conjugating enzyme UBC9 antibody
    • SUMO-protein ligase antibody
    • SUMO1 protein ligase antibody
    • UBC9 antibody
    • UBC9_HUMAN antibody
    • UBCE9 antibody
    • Ube2i antibody
    • Ubiquitin carrier protein 9 antibody
    • Ubiquitin carrier protein antibody
    • Ubiquitin carrier protein I antibody
    • Ubiquitin conjugating enzyme 9 antibody
    • Ubiquitin conjugating enzyme E2I (homologous to yeast UBC9) antibody
    • Ubiquitin conjugating enzyme E2I (UBC9 homolog, yeast) antibody
    • Ubiquitin conjugating enzyme UbcE2A antibody
    • Ubiquitin like protein SUMO 1 conjugating enzyme antibody
    • Ubiquitin protein ligase E2I antibody
    • Ubiquitin-conjugating enzyme E2 I antibody
    • Ubiquitin-protein ligase I antibody
    see all


  • Anti-UBE2I / UBC9 antibody (ab21193) at 0.3 µg/ml + Rat ovary lysate at 35 µg

    Predicted band size: 18 kDa

  • ab21193 staining UBE2I / UBC9 in mouse oocyte by Immunocytochemistry. The cells were fixed in paraformaldehyde, permeabilized in 0.1% Tween and blocked in 0.1% BSA for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with sample for 1 hour at 25°C. A Cy5® conjugated Donkey polyclonal to goat, diluted 1/400 was used as the secondary.

    See Abreview

  • Ab21193 (0.3ug/ml) staining human UBE2I in human kidney by immunohistochemistry using paraffin embedded tissue.

    Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

  • Anti-UBE2I / UBC9 antibody (ab21193) at 0.1 µg/ml + Human kidney lysate at 35 µg

    Predicted band size: 18 kDa


This product has been referenced in:
  • Fang S  et al. Down-regulation of UBC9 increases the sensitivity of hepatocellular carcinoma to doxorubicin. Oncotarget 8:49783-49795 (2017). Read more (PubMed: 28572537) »
  • Xu J  et al. BRCA1 Mutation Leads to Deregulated Ubc9 Levels which Triggers Proliferation and Migration of Patient-Derived High Grade Serous Ovarian Cancer and Triple Negative Breast Cancer Cells. Int J Chronic Dis Ther 2:31-38 (2016). WB . Read more (PubMed: 28164176) »
See all 9 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - whole cell (Human fibroblast)
Loading amount
10 µg
Human fibroblast
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Nupage)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 16 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunocytochemistry/ Immunofluorescence
Mouse Cell (oocyte, embryo)
oocyte, embryo
Yes - 0.1% Triton
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 25°C

Dr. Motomasa Ihara

Verified customer

Submitted Nov 04 2008


Thank you for your enquiry. All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as we receive additional data. The information you have requested for can be found from the datasheet: 1) "Tested applications" - ELISA, Immunohistochemistry (Paraffin-embedded sections), Western blot. This product has not been tested in ICC and it may or may not work in this application. We do not guarantee that ab21193 will work in untested applications. 2) "Species Reactivity" - Reacts with Human. Not yet tested in other species. Predicted to react with Mouse (100% identity with immunogen), Rat (100% identity with immunogen), Xenopus laevis (100% identity with immunogen), Chicken (100% identity with immunogen) and Hamster due to sequence homology. This product reacts with Human but has not been tested on Rat. Based on the immunogen sequence, ab21193 is predicted to crossreact with rat but we do not guarantee this. 3) "Availability" - Select United Kingdom and click on "view price for this country". Please don't hesitate to contact us again if you have further questions.

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