• Product name
    Anti-Ube2L3 antibody [EPR4368]
    See all Ube2L3 primary antibodies
  • Description
    Rabbit monoclonal [EPR4368] to Ube2L3
  • Host species
  • Tested applications
    Suitable for: ICC/IF, WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ube2L3. The exact sequence is proprietary.

  • Positive control
    • WB: HeLa, LNCaP, Jurkat and K562 cell lysates IHC-P: Human testis tissue IF: HeLa cells
  • General notes



    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab108936 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Predicted molecular weight: 18 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
    • Tissue specificity
      Ubiquitous, with highest expression in testis.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the ubiquitin-conjugating enzyme family.
    • Post-translational
      Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
    • Cellular localization
      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • E2 F1 antibody
      • L UBC antibody
      • L-UBC antibody
      • UB2L3_HUMAN antibody
      • UBCE7 antibody
      • UbcH7 antibody
      • UbcM4 antibody
      • Ube2l3 antibody
      • Ubiquitin carrier protein L3 antibody
      • Ubiquitin conjugating enzyme E2 L3 antibody
      • Ubiquitin protein ligase L3 antibody
      • Ubiquitin-conjugating enzyme E2 L3 antibody
      • Ubiquitin-conjugating enzyme E2-F1 antibody
      • Ubiquitin-protein ligase L3 antibody
      see all


    • All lanes : Anti-Ube2L3 antibody [EPR4368] (ab108936) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : LNCaP cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 18 kDa

    • ab108936, at a 1/100 dilution, staining Ube2L3 in paraffin-embedded Human testis tissue
    • ab108936, at a 1/100 dilution, staining Ube2L3 in HeLa cells


    This product has been referenced in:
    • Ma X  et al. Ubiquitin conjugating enzyme E2 L3 promoted tumor growth of NSCLC through accelerating p27kip1 ubiquitination and degradation. Oncotarget 8:84193-84203 (2017). Read more (PubMed: 29137415) »
    • Wang C  et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). Read more (PubMed: 27082114) »

    See all 2 Publications for this product

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