• Product name

    Anti-Ube2L3/UBCH7 antibody
    See all Ube2L3/UBCH7 primary antibodies
  • Description

    Rabbit polyclonal to Ube2L3/UBCH7
  • Host species

  • Tested applications

    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human Ube2L3/UBCH7 aa 100-154 (C terminal).
    Database link: NP_003338.1

  • Positive control

    • HeLa, 293T and NIH3T3 whole cell lysates.
  • General notes

     This product was previously labelled as Ube2L3




Our Abpromise guarantee covers the use of ab72545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
IP Use at 2-5 µg/mg of lysate.

Efficiency in IP is low.


  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
  • Tissue specificity

    Ubiquitous, with highest expression in testis.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational

    Ubiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • E2 F1 antibody
    • L UBC antibody
    • L-UBC antibody
    • UB2L3_HUMAN antibody
    • UBCE7 antibody
    • UbcH7 antibody
    • UbcM4 antibody
    • Ube2l3 antibody
    • Ubiquitin carrier protein L3 antibody
    • Ubiquitin conjugating enzyme E2 L3 antibody
    • Ubiquitin protein ligase L3 antibody
    • Ubiquitin-conjugating enzyme E2 L3 antibody
    • Ubiquitin-conjugating enzyme E2-F1 antibody
    • Ubiquitin-protein ligase L3 antibody
    see all


  • All lanes : Anti-Ube2L3/UBCH7 antibody (ab72545) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Predicted band size: 18 kDa
    Observed band size: 18 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Ube2L3/UBCH7 with ab72545 at 1/1000 (1µg/ml). Detection: DAB.

  • Detection of Human Ube2L3/UBCH7 by Immunoprecipitation from Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab72545 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.


ab72545 has not yet been referenced specifically in any publications.

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