Product nameAnti-Ube2L3/UBCH7 antibody [EPR4368]
See all Ube2L3/UBCH7 primary antibodies
DescriptionRabbit monoclonal [EPR4368] to Ube2L3/UBCH7
Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Ube2L3/UBCH7. The exact sequence is proprietary.
- WB: HeLa, LNCaP, Jurkat and K562 cell lysates IHC-P: Human testis tissue IF: HeLa cells
This product was previously labelled as Ube2L3
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab108936 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/100 - 1/250.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 18 kDa.|
|IP||1/10 - 1/100.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.|
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
Tissue specificityUbiquitous, with highest expression in testis.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
modificationsUbiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- E2 F1 antibody
- L UBC antibody
- L-UBC antibody
All lanes : Anti-Ube2L3/UBCH7 antibody [EPR4368] (ab108936) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 18 kDa
ab108936, at a 1/100 dilution, staining Ube2L3/UBCH7 in paraffin-embedded Human testis tissue
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ab108936, at a 1/100 dilution, staining Ube2L3/UBCH7 in HeLa cells
This product has been referenced in:
- Liu Y et al. UBE2L3, a susceptibility gene that plays oncogenic role in hepatitis B-related hepatocellular carcinoma. J Viral Hepat N/A:N/A (2018). Read more (PubMed: 29969176) »
- Ma X et al. Ubiquitin conjugating enzyme E2 L3 promoted tumor growth of NSCLC through accelerating p27kip1 ubiquitination and degradation. Oncotarget 8:84193-84203 (2017). Read more (PubMed: 29137415) »