Overview

  • Product name

    Anti-Ube2N / Ubc13 antibody
    See all Ube2N / Ubc13 primary antibodies
  • Description

    Rabbit polyclonal to Ube2N / Ubc13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Sheep, Chicken, Cow, Dog, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Ube2N/ Ubc13 (internal sequence). The exact sequence is proprietary.
    Database link: P61088

  • Positive control

    • WB: Mouse brain whole cell lysate; A431 and HepG2 whole cell lysates. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab228744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 17 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications

    Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • Bendless like ubiquitin conjugating enzyme antibody
    • Bendless-like ubiquitin-conjugating enzyme antibody
    • BLU antibody
    • EC 6.3.2.19 antibody
    • Epididymis secretory protein Li 71 antibody
    • HEL-S-71 antibody
    • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
    • MGC131857 antibody
    • MGC8489 antibody
    • UBC 13 antibody
    • Ubc13 antibody
    • UbCH ben antibody
    • UbcH-ben antibody
    • UbcH13 antibody
    • UBCHBEN antibody
    • Ube 2N antibody
    • Ube2n antibody
    • UBE2N_HUMAN antibody
    • Ubiquitin carrier protein N antibody
    • Ubiquitin conjugating enzyme E2 N antibody
    • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
    • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2N antibody
    • Ubiquitin protein ligase N antibody
    • Ubiquitin-conjugating enzyme E2 N antibody
    • Ubiquitin-protein ligase N antibody
    • Yeast UBC13 homolog antibody
    see all

Images

  • HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ube2N / Ubc13 (green) using ab228744 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Nuclear counterstain: Hoechst 33342 (blue).

  • Anti-Ube2N / Ubc13 antibody (ab228744) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 17 kDa



    12% SDS-PAGE

  • All lanes : Anti-Ube2N / Ubc13 antibody (ab228744) at 1/1000 dilution

    Lane 1 : A431 (human epidermoid carcinoma cell line) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 17 kDa



    12% SDS-PAGE

References

ab228744 has not yet been referenced specifically in any publications.

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