Anti-Ube2N / Ubc13 antibody (ab25885)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Ube2N / Ubc13 antibody
See all Ube2N / Ubc13 primary antibodies -
DescriptionRabbit polyclonal to Ube2N / Ubc13
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide, corresponding to 15 amino acids near the C terminus of Human Ube2N.
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Positive control
- Human small intestine or HepG2 cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
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Concentration information loading... -
Purification notesThis antibody was purified by Ion exchange chromatography.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab25885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 18 kDa. | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use a concentration of 2 µg/ml. |
Target
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FunctionThe UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
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PathwayProtein modification; protein ubiquitination.
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Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
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Post-translational
modificationsConjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2. -
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 7334 Human
- Entrez Gene: 93765 Mouse
- Entrez Gene: 116725 Rat
- Omim: 603679 Human
- SwissProt: P61088 Human
- SwissProt: P61089 Mouse
- SwissProt: Q9EQX9 Rat
- Unigene: 524630 Human
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Alternative names
- Bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
see all
Images
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Western blot - Anti-Ube2N / Ubc13 antibody (ab25885)All lanes : Anti-Ube2N / Ubc13 antibody (ab25885) (analysis
in human
small intestine cell lysates 15ug/lane
)
Lane 1 : 0.5 ug/ml of antibody.
Lane 2 : 1 ug/ml of antibody.
Lane 3 : 2 ug/ml of antibody.
Predicted band size: 18 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube2N / Ubc13 antibody (ab25885)ab25885 at 2µg/ml staining UBe2N in mouse thymus tissue by IHC
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Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (ab25885)ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Zhang X et al. The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer. Cell Death Dis 9:93 (2018). Read more (PubMed: 29367628) »
- Maculins T et al. A Generic Platform for Cellular Screening Against Ubiquitin Ligases. Sci Rep 6:18940 (2016). Read more (PubMed: 26743172) »
