• Product name
    Anti-Ube2N / Ubc13 antibody
    See all Ube2N / Ubc13 primary antibodies
  • Description
    Rabbit polyclonal to Ube2N / Ubc13
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to 15 amino acids near the C terminus of Human Ube2N.

  • Positive control
    • Human small intestine or HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab25885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 18 kDa.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 2 µg/ml.


  • Function
    The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Bendless like ubiquitin conjugating enzyme antibody
    • Bendless-like ubiquitin-conjugating enzyme antibody
    • BLU antibody
    • EC antibody
    • Epididymis secretory protein Li 71 antibody
    • HEL-S-71 antibody
    • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
    • MGC131857 antibody
    • MGC8489 antibody
    • UBC 13 antibody
    • Ubc13 antibody
    • UbCH ben antibody
    • UbcH-ben antibody
    • UbcH13 antibody
    • UBCHBEN antibody
    • Ube 2N antibody
    • Ube2n antibody
    • UBE2N_HUMAN antibody
    • Ubiquitin carrier protein N antibody
    • Ubiquitin conjugating enzyme E2 N antibody
    • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
    • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2N antibody
    • Ubiquitin protein ligase N antibody
    • Ubiquitin-conjugating enzyme E2 N antibody
    • Ubiquitin-protein ligase N antibody
    • Yeast UBC13 homolog antibody
    see all


  • All lanes : Anti-Ube2N / Ubc13 antibody (ab25885) (analysis
    in human
    small intestine cell lysates 15ug/lane

    Lane 1 : 0.5 ug/ml of antibody.
    Lane 2 : 1 ug/ml of antibody.
    Lane 3 : 2 ug/ml of antibody.

    Predicted band size: 18 kDa

  • ab25885 at 2µg/ml staining UBe2N in mouse thymus tissue by IHC

  • ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Zhang X  et al. The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer. Cell Death Dis 9:93 (2018). Read more (PubMed: 29367628) »
  • Maculins T  et al. A Generic Platform for Cellular Screening Against Ubiquitin Ligases. Sci Rep 6:18940 (2016). Read more (PubMed: 26743172) »
See all 4 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Tissue lysate - whole (stomach)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Jul 11 2011


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