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    ube2n--ubc13-antibody-ab25885.pdf

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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation
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Anti-Ube2N / Ubc13 antibody (ab25885)

  • Datasheet
Reviews (1) Submit a question References (4)

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Western blot - Anti-Ube2N / Ubc13 antibody (ab25885)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (ab25885)

Key features and details

  • Rabbit polyclonal to Ube2N / Ubc13
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Ube2N / Ubc13 antibody
    See all Ube2N / Ubc13 primary antibodies
  • Description

    Rabbit polyclonal to Ube2N / Ubc13
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human small intestine cell lysate. ICC: Hep G2 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
  • Concentration information loading...
  • Purification notes

    This antibody was purified by Ion exchange chromatography.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Homologous Recomb.
    • Immunology
    • Innate Immunity
    • TLR Signaling
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E2 Ubiquitin Conjugating Enzymes
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E2 Enzymes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Ube2N / Ubc13 protein (ab95900)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab25885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 18 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 18 kDa.
IHC-P
Use at an assay dependent concentration.
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications

    Conjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Target information above from: UniProt accession P61088 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7334 Human
    • Entrez Gene: 93765 Mouse
    • Entrez Gene: 116725 Rat
    • Omim: 603679 Human
    • SwissProt: P61088 Human
    • SwissProt: P61089 Mouse
    • SwissProt: Q9EQX9 Rat
    • Unigene: 524630 Human
    • Unigene: 371667 Mouse
    • Unigene: 165094 Rat
    see all
  • Alternative names

    • Bendless like ubiquitin conjugating enzyme antibody
    • Bendless-like ubiquitin-conjugating enzyme antibody
    • BLU antibody
    • EC 6.3.2.19 antibody
    • Epididymis secretory protein Li 71 antibody
    • HEL-S-71 antibody
    • Human epidermoid carcinoma mRNA for ubiquitin-conjugating enzyme E2 similar to Drosophila bendless gene product complete cds antibody
    • MGC131857 antibody
    • MGC8489 antibody
    • UBC 13 antibody
    • Ubc13 antibody
    • UbCH ben antibody
    • UbcH-ben antibody
    • UbcH13 antibody
    • UBCHBEN antibody
    • Ube 2N antibody
    • Ube2n antibody
    • UBE2N_HUMAN antibody
    • Ubiquitin carrier protein N antibody
    • Ubiquitin conjugating enzyme E2 N antibody
    • Ubiquitin conjugating enzyme E2N (homologous to yeast UBC13) antibody
    • Ubiquitin conjugating enzyme E2N (UBC13 homolog yeast) antibody
    • Ubiquitin conjugating enzyme E2N antibody
    • Ubiquitin protein ligase N antibody
    • Ubiquitin-conjugating enzyme E2 N antibody
    • Ubiquitin-protein ligase N antibody
    • Yeast UBC13 homolog antibody
    see all

Images

  • Western blot - Anti-Ube2N / Ubc13 antibody (ab25885)
    Western blot - Anti-Ube2N / Ubc13 antibody (ab25885)
    All lanes : Anti-Ube2N / Ubc13 antibody (ab25885) (analysis
    in human
    small intestine cell lysates 15ug/lane
    )

    Lane 1 : 0.5 ug/ml of antibody.
    Lane 2 : 1 ug/ml of antibody.
    Lane 3 : 2 ug/ml of antibody.

    Predicted band size: 18 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (ab25885)
    Immunocytochemistry/ Immunofluorescence - Anti-Ube2N / Ubc13 antibody (ab25885)
    ICC/IF image of ab25885 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab25885, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (4)

Publishing research using ab25885? Please let us know so that we can cite the reference in this datasheet.

ab25885 has been referenced in 4 publications.

  • Zhang X  et al. The inhibition of UBC13 expression and blockage of the DNMT1-CHFR-Aurora A pathway contribute to paclitaxel resistance in ovarian cancer. Cell Death Dis 9:93 (2018). PubMed: 29367628
  • Maculins T  et al. A Generic Platform for Cellular Screening Against Ubiquitin Ligases. Sci Rep 6:18940 (2016). PubMed: 26743172
  • Nakada S  et al. RNF8 regulates assembly of RAD51 at DNA double-strand breaks in the absence of BRCA1 and 53BP1. Cancer Res 72:4974-83 (2012). PubMed: 22865450
  • Kocevar N  et al. Proteomic analysis of gastric cancer and immunoblot validation of potential biomarkers. World J Gastroenterol 18:1216-28 (2012). WB ; Human . PubMed: 22468085

Customer reviews and Q&As

Show All Reviews Q&A
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Western blot abreview for Anti-Ube2N / Ubc13 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Tissue lysate - whole (stomach)
Loading amount
30 µg
Specification
stomach
Gel Running Conditions
Reduced Denaturing (12 %)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 11 2011

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