Product nameAnti-Ube2N / Ubc13 antibody [EPR5162]
See all Ube2N / Ubc13 primary antibodies
DescriptionRabbit monoclonal [EPR5162] to Ube2N / Ubc13
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Ube2N/ Ubc13 aa 1-100. The exact sequence is proprietary.
- Human testis tissue. Jurkat, Daudi and Human heart lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
- Epigenetics and Nuclear Signaling
- Ubiquitin & Ubiquitin Like Modifiers
- E2 Ubiquitin Conjugating Enzymes
Our Abpromise guarantee covers the use of ab109286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 17 kDa.|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/250.|
FunctionThe UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains. This type of polyubiquitination does not lead to protein degradation by the proteasome. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair. Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
modificationsConjugation to ISG15 impairs formation of the thioester bond with ubiquitin but not interaction with UBE2V2.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- Bendless like ubiquitin conjugating enzyme antibody
- Bendless-like ubiquitin-conjugating enzyme antibody
- BLU antibody
All lanes : Anti-Ube2N / Ubc13 antibody [EPR5162] (ab109286) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Human heart lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 17 kDa
Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab109286 at 1/100 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
This product has been referenced in:
- Morale MG et al. HPV-transformed cells exhibit altered HMGB1-TLR4/MyD88-SARM1 signaling axis. Sci Rep 8:3476 (2018). WB . Read more (PubMed: 29472602) »
- Hu L et al. Oligomerization-primed coiled-coil domain interaction with Ubc13 confers processivity to TRAF6 ubiquitin ligase activity. Nat Commun 8:814 (2017). Read more (PubMed: 28993672) »