Key features and details
- Rabbit polyclonal to UBE2Q2
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-UBE2Q2 antibody
See all UBE2Q2 primary antibodies
DescriptionRabbit polyclonal to UBE2Q2
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human UBE2Q2 aa 325-375. The exact sequence is proprietary. (NP_775740.1).
Database link: Q8WVN8
- WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab225869 in the following tested applications.
|WB||1/2000 - 1/10000.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.
Tissue specificityDetected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
- LOC92912 antibody
- UB2Q2_HUMAN antibody
- UBE2Q2 antibody
All lanes : Anti-UBE2Q2 antibody (ab225869) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line0 whole cell lysate at 50 µg
Developed using the ECL technique.
Exposure time: 30 seconds
UBE2Q2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225869 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225869 at 0.4 µg/ml.
Lane 1: ab225869 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab225869 has not yet been referenced specifically in any publications.