Overview

  • Product name

  • Description

    Rabbit polyclonal to UBE2Q2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human UBE2Q2 aa 325-375. The exact sequence is proprietary. (NP_775740.1).
    Database link: Q8WVN8

  • Positive control

    • WB: HeLa, HEK-293T and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab225869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination.
  • Tissue specificity

    Detected in hypopharyngeal head and neck squamous cell carcinoma, in tumor masses and invasive epithelium.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • LOC92912 antibody
    • UB2Q2_HUMAN antibody
    • UBE2Q2 antibody
    • Ubiquitin carrier protein Q2 antibody
    • Ubiquitin conjugating enzyme E2Q 2 antibody
    • Ubiquitin conjugating enzyme E2Q family member 2 antibody
    • Ubiquitin-conjugating enzyme E2 Q2 antibody
    • ubiquitin-conjugating enzyme E2Q (putative) 2 antibody
    • Ubiquitin-protein ligase Q2 antibody
    see all

Images

  • All lanes : Anti-UBE2Q2 antibody (ab225869) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line0 whole cell lysate at 50 µg

    Developed using the ECL technique.

    Exposure time: 30 seconds
  • UBE2Q2 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225869 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab225869 at 0.4 µg/ml.

    Lane 1: ab225869 IP in HeLa whole cell lysate.
    Lane 2: Control IgG IP in HeLa whole cell lysate.


    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab225869 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab225869.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up