Overview

  • Product name

  • Description

    Rabbit polyclonal to UBE2S
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human UBE2S aa 172-222. The exact sequence is proprietary. (NP_055316.2)
    Sequence:

    ASGTEASST DPGAPGGPGG AEGPMAKKHA GERDKKLAAK KKTDKKRALR RL


    Database link: Q16763

  • Positive control

    • 293T, HeLa, Jurkat, mouse TCMK1 and mouse NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab177508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 24 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications

    Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA409170 antibody
    • E2 EPF antibody
    • E2-EPF antibody
    • E2EPF antibody
    • EPF5 antibody
    • MGC101982 antibody
    • Ube2s antibody
    • UBE2S_HUMAN antibody
    • Ubiquitin carrier protein S antibody
    • Ubiquitin conjugating enzyme E2 24 kD antibody
    • Ubiquitin conjugating enzyme E2 24 kDa antibody
    • Ubiquitin conjugating enzyme E2 EPF5 antibody
    • Ubiquitin conjugating enzyme E2 S antibody
    • Ubiquitin conjugating enzyme E2S antibody
    • Ubiquitin protein ligase S antibody
    • Ubiquitin-conjugating enzyme E2 S antibody
    • Ubiquitin-conjugating enzyme E2-24 kDa antibody
    • Ubiquitin-conjugating enzyme E2-EPF5 antibody
    • Ubiquitin-protein ligase S antibody
    see all

Images

  • All lanes : Anti-UBE2S antibody (ab177508) at 0.1 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : mouse TCMK1 whole cell lysate
    Lane 5 : mouse NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 24 kDa


    Exposure time: 3 minutes
  • Detection of UBE2S in Immunoprecipitates of 293Twhole cell lysates (1 mg for IP, 20% of IP loaded) using ab177508 at 6 µg/mg lysate for IP (Lane 1). For WB detection, ab177508 was used at 1 µg/ml. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 10 seconds.

References

This product has been referenced in:

  • Yau RG  et al. Assembly and Function of Heterotypic Ubiquitin Chains in Cell-Cycle and Protein Quality Control. Cell 171:918-933.e20 (2017). Read more (PubMed: 29033132) »
See 1 Publication for this product

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