Overview

  • Product name

  • Description

    Rabbit polyclonal to UBE2S
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Xenopus laevis, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human UBE2S (internal sequence). The exact sequence is proprietary.
    Database link: Q16763

  • Positive control

    • WB: HCT 116 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab228848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 24 kDa.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro; it is however unclear whether UBE2D1/UBCH5 acts as a E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.
  • Pathway

    Protein modification; protein ubiquitination.
  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Post-translational
    modifications

    Autoubiquitinated by the APC/C complex during G1, leading to its degradation by the proteasome.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA409170 antibody
    • E2 EPF antibody
    • E2-EPF antibody
    • E2EPF antibody
    • EPF5 antibody
    • MGC101982 antibody
    • Ube2s antibody
    • UBE2S_HUMAN antibody
    • Ubiquitin carrier protein S antibody
    • Ubiquitin conjugating enzyme E2 24 kD antibody
    • Ubiquitin conjugating enzyme E2 24 kDa antibody
    • Ubiquitin conjugating enzyme E2 EPF5 antibody
    • Ubiquitin conjugating enzyme E2 S antibody
    • Ubiquitin conjugating enzyme E2S antibody
    • Ubiquitin protein ligase S antibody
    • Ubiquitin-conjugating enzyme E2 S antibody
    • Ubiquitin-conjugating enzyme E2-24 kDa antibody
    • Ubiquitin-conjugating enzyme E2-EPF5 antibody
    • Ubiquitin-protein ligase S antibody
    see all

Images

  • All lanes : Anti-UBE2S antibody (ab228848) at 1/2000 dilution

    Lane 1 : WT 293T cell extracts
    Lane 2 : UBE2S Knockout 293T cell extracts

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 24 kDa

  • Anti-UBE2S antibody (ab228848) at 1/1000 dilution + HCT 116 (human colorectal carcinoma cell line) whole cell lysate at 30 µg

    Developed using the ECL technique.

    Predicted band size: 24 kDa



    12% SDS-PAGE

References

ab228848 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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