Product nameAnti-UBE2U antibody
DescriptionRabbit polyclonal to UBE2U
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human UBE2U aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Liver (Human) Tissue Lysate - adult normal tissue;
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab76653 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).|
|ICC/IF||Use at an assay dependent concentration.|
FunctionCatalyzes the covalent attachment of ubiquitin to other proteins.
PathwayProtein modification; protein ubiquitination.
Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
- Information by UniProt
- RP4-636O23.1 antibody
- UBE2U antibody
- UBE2U_HUMAN antibody
Anti-UBE2U antibody (ab76653) at 1/250 dilution + Human liver tissue lysate - total protein (ab29889) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 38 kDa
Observed band size: 38 kDa
Exposure time: 20 minutes
ICC/IF image of ab76653 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76653, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab76653 has not yet been referenced specifically in any publications.