Recombinant Anti-UBE2V1 antibody [EPR4735(2)] (ab151725)
![Western blot - Anti-UBE2V1 antibody [EPR4735(2)] (ab151725)](https://www.abcam.com/ps/products/151/ab151725/Images/ab151725-158796-anti-ube2v1-antibody-epr47352-western-blot.jpg "Western blot - Anti-UBE2V1 antibody [EPR4735(2)] (ab151725)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4735(2)] to UBE2V1
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-UBE2V1 antibody [EPR4735(2)]
See all UBE2V1 primary antibodies -
Description
Rabbit monoclonal [EPR4735(2)] to UBE2V1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, HT29, Human fetal kidney and BxPC3 lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR4735(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151725 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
Target
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Function
Has no ubiquitin ligase activity on its own. The UBE2V1-UBE2N heterodimer catalyzes the synthesis of non-canonical poly-ubiquitin chains that are linked through Lys-63. This type of poly-ubiquitination activates IKK and does not seem to involve protein degradation by the proteasome. Plays a role in the activation of NF-kappa-B mediated by IL1B, TNF, TRAF6 and TRAF2. Mediates transcriptional activation of target genes. Plays a role in the control of progress through the cell cycle and differentiation. Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage. -
Tissue specificity
Highly expressed in thyroid, pancreas, spinal cord, lymph node, trachea, adrenal gland, bone marrow and pancreas. Detected at low levels in heart, breast, placenta, brain, liver, kidney, stomach and lung. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Cellular localization
Nucleus. Excluded from the nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 387522 Human
- Entrez Gene: 7335 Human
- Entrez Gene: 66589 Mouse
- Entrez Gene: 296390 Rat
- Omim: 602995 Human
- SwissProt: Q13404 Human
- SwissProt: Q9CZY3 Mouse
- Unigene: 420529 Human
see all -
Alternative names
- CIR1 antibody
- CROC-1 antibody
- CROC1 antibody
see all
Images
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Western blot - Anti-UBE2V1 antibody [EPR4735(2)] (ab151725)All lanes : Anti-UBE2V1 antibody [EPR4735(2)] (ab151725) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : HT29 cell lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : BxPC3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 16 kDa -
![Anti-UBE2V1 antibody [EPR4735(2)] (ab151725)](https://www.abcam.com/ps/products/151/ab151725/Images/ab151725-2-benefits-of-recombinant-antibodies.png)
Anti-UBE2V1 antibody [EPR4735(2)] (ab151725)
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab151725 has been referenced in 2 publications.
- Li H et al. Linc00426 accelerates lung adenocarcinoma progression by regulating miR-455-5p as a molecular sponge. Cell Death Dis 11:1051 (2020). PubMed: 33311443
- Shen T et al. Ube2v1-mediated ubiquitination and degradation of Sirt1 promotes metastasis of colorectal cancer by epigenetically suppressing autophagy. J Hematol Oncol 11:95 (2018). PubMed: 30016968