Anti-UBE2Z antibody (ab229877)
Key features and details
- Rabbit polyclonal to UBE2Z
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBE2Z antibody
See all UBE2Z primary antibodies -
Description
Rabbit polyclonal to UBE2Z -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment corresponding to Human UBE2Z aa 100 to the C-terminus.
Database link: Q9H832 -
Positive control
- WB: MCF7, HepG2 and HeLa whole cell lysates. IHC-P: Human testis and brain tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa).
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IHC-P |
1/20 - 1/200.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 38 kDa (predicted molecular weight: 39 kDa). |
IHC-P
1/20 - 1/200. |
Target
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Function
Catalyzes the covalent attachment of ubiquitin to other proteins (By similarity). Specific substrate for UBE1L2, not charged with ubiquitin by UBE1. May be involved in apoptosis regulation. -
Tissue specificity
Widely expressed. Highly in placenta, pancreas, spleen and testis. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 65264 Human
- Entrez Gene: 268470 Mouse
- Entrez Gene: 303478 Rat
- Omim: 611362 Human
- SwissProt: Q9H832 Human
- SwissProt: Q3UE37 Mouse
- SwissProt: Q3B7D1 Rat
- Unigene: 514297 Human
see all -
Alternative names
- FLJ13855 antibody
- GC17P044341 antibody
- HOYS7 antibody
see all
Images
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All lanes : Anti-UBE2Z antibody (ab229877) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Secondary
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
Paraffin-embedded human testis tissue stained for UBE2Z using ab229877 at 1/100 dilution in immunohistochemical analysis.
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Paraffin-embedded human brain tissue stained for UBE2Z using ab229877 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab229877 has not yet been referenced specifically in any publications.