• Product name
  • Description
    Rabbit polyclonal to UBE3A
  • Host species
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Chicken, Guinea pig, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
  • Immunogen

    Immunogen was a synthetic peptide, which represented a portion of human ubiquitin protein ligase E3A encoded within exon 7 (LocusLink ID 7337).

  • Positive control
    • WB: T24/83 and HepG2 cell lysate.



Our Abpromise guarantee covers the use of ab10488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
IP Use at 1-4 µg/mg of lysate.


  • Function
    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
  • Pathway
    Protein modification; protein ubiquitination.
  • Involvement in disease
    Angelman syndrome
  • Sequence similarities
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
  • Post-translational
    Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANCR antibody
    • Angelman syndrome antibody
    • AS antibody
    • CTCL tumor antigen se37 2 antibody
    • CTCL tumor antigen se37-2 antibody
    • E6 AP antibody
    • E6AP antibody
    • E6AP ubiquitin protein ligase antibody
    • E6AP ubiquitin-protein ligase antibody
    • EPVE6AP antibody
    • FLJ26981 antibody
    • HECT-type ubiquitin transferase E3A antibody
    • HPVE6A antibody
    • Human papilloma virus E6 associated protein Angelman syndrome antibody
    • Human papilloma virus E6 associated protein antibody
    • Human papillomavirus E6-associated protein antibody
    • NY REN 54 antigen antibody
    • Oncogenic protein associated protein E6 AP antibody
    • Oncogenic protein associated protein E6AP antibody
    • Oncogenic protein-associated protein E6-AP antibody
    • Renal carcinoma antigen NY REN 54 antibody
    • Renal carcinoma antigen NY-REN-54 antibody
    • UBE 3A antibody
    • Ube3a antibody
    • UBE3A protein antibody
    • UBE3A_HUMAN antibody
    • Ubiquitin protein ligase E3A antibody
    • Ubiquitin-protein ligase E3A antibody
    see all


  • All lanes : Anti-UBE3A antibody (ab10488) at 1/1000 dilution

    Lane 1 : T24/83 (Human bladder carcinoma) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size: 105 kDa
    Observed band size: 105 kDa
    Additional bands at: 120 kDa, 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands.


This product has been referenced in:
See 1 Publication for this product

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