Anti-UBE3A antibody (ab10488)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-UBE3A antibody
See all UBE3A primary antibodies -
DescriptionRabbit polyclonal to UBE3A
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Chicken, Guinea pig, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis, Medaka fish
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Immunogen
Immunogen was a synthetic peptide, which represented a portion of human ubiquitin protein ligase E3A encoded within exon 7 (LocusLink ID 7337).
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Positive control
- WB: T24/83 and HepG2 cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesAntibodies were affinity purified using the peptide immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab10488 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa). | |
| IP | Use at 1-4 µg/mg of lysate. |
Target
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FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
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PathwayProtein modification; protein ubiquitination.
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Involvement in diseaseAngelman syndrome
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Sequence similaritiesContains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
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Post-translational
modificationsPhosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells. -
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
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Database links
- Entrez Gene: 7337 Human
- Entrez Gene: 22215 Mouse
- Omim: 601623 Human
- SwissProt: Q05086 Human
- SwissProt: O08759 Mouse
- Unigene: 598862 Human
- Unigene: 9002 Mouse
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Alternative names
- ANCR antibody
- Angelman syndrome antibody
- AS antibody
see all
Images
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Western blot - Anti-UBE3A antibody (ab10488)All lanes : Anti-UBE3A antibody (ab10488) at 1/1000 dilution
Lane 1 : T24/83 (Human bladder carcinoma) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 105 kDa
Observed band size: 105 kDa
Additional bands at: 120 kDa, 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
References
This product has been referenced in:
- Lawson-Yuen A et al. Ube3a mRNA and protein expression are not decreased in Mecp2R168X mutant mice. Brain Res 1180:1-6 (2007). Read more (PubMed: 17936729) »
