For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    ube3a-antibody-ab10488.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation
Share by email

Anti-UBE3A antibody (ab10488)

  • Datasheet
  • SDS
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-UBE3A antibody (ab10488)

    Key features and details

    • Rabbit polyclonal to UBE3A
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human UBE3A protein (ab206157)

    View more associated products

    Overview

    • Product name

      Anti-UBE3A antibody
      See all UBE3A primary antibodies
    • Description

      Rabbit polyclonal to UBE3A
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Immunogen was a synthetic peptide, which represented a portion of human ubiquitin protein ligase E3A encoded within exon 7 (LocusLink ID 7337).

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Antibodies were affinity purified using the peptide immobilized on solid support.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Ubiquitylation
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ubiquitin E3 Enzymes
      • Hect E3 Ligase
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Co-activators/co-repressors

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human UBE3A protein (ab206157)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab10488 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Rabbit
    Chicken
    Guinea pig
    Turkey
    Pig
    Xenopus laevis
    Chimpanzee
    Rhesus monkey
    Gorilla
    Tilapia
    Orangutan
    Xenopus tropicalis
    Medaka fish
    Application Abreviews Notes
    WB
    1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).
    Notes
    WB
    1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).

    Target

    • Function

      E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
    • Pathway

      Protein modification; protein ubiquitination.
    • Involvement in disease

      Angelman syndrome
    • Sequence similarities

      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    • Post-translational
      modifications

      Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Target information above from: UniProt accession Q05086 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 7337 Human
      • Omim: 601623 Human
      • SwissProt: Q05086 Human
      • Unigene: 598862 Human
      • Alternative names

        • ANCR antibody
        • Angelman syndrome antibody
        • AS antibody
        • CTCL tumor antigen se37 2 antibody
        • CTCL tumor antigen se37-2 antibody
        • E6 AP antibody
        • E6AP antibody
        • E6AP ubiquitin protein ligase antibody
        • E6AP ubiquitin-protein ligase antibody
        • EPVE6AP antibody
        • FLJ26981 antibody
        • HECT-type ubiquitin transferase E3A antibody
        • HPVE6A antibody
        • Human papilloma virus E6 associated protein Angelman syndrome antibody
        • Human papilloma virus E6 associated protein antibody
        • Human papillomavirus E6-associated protein antibody
        • NY REN 54 antigen antibody
        • Oncogenic protein associated protein E6 AP antibody
        • Oncogenic protein associated protein E6AP antibody
        • Oncogenic protein-associated protein E6-AP antibody
        • Renal carcinoma antigen NY REN 54 antibody
        • Renal carcinoma antigen NY-REN-54 antibody
        • UBE 3A antibody
        • Ube3a antibody
        • UBE3A protein antibody
        • UBE3A_HUMAN antibody
        • Ubiquitin protein ligase E3A antibody
        • Ubiquitin-protein ligase E3A antibody
        see all

      Images

      • Western blot - Anti-UBE3A antibody (ab10488)
        Western blot - Anti-UBE3A antibody (ab10488)
        All lanes : Anti-UBE3A antibody (ab10488) at 1/1000 dilution

        Lane 1 : T24/83 (Human bladder carcinoma) Whole Cell Lysate
        Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Predicted band size: 105 kDa
        Observed band size: 105 kDa
        Additional bands at: 120 kDa, 30 kDa, 75 kDa. We are unsure as to the identity of these extra bands.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (1)

      Publishing research using ab10488? Please let us know so that we can cite the reference in this datasheet.

      ab10488 has been referenced in 1 publication.

      • Lawson-Yuen A  et al. Ube3a mRNA and protein expression are not decreased in Mecp2R168X mutant mice. Brain Res 1180:1-6 (2007). PubMed: 17936729

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      There are currently no Customer reviews or Questions for ab10488.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2021 Abcam plc. All rights reserved.