Overview

  • Product name

  • Description

    Rabbit polyclonal to UBE3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length protein (proprietary-tag) corresponding to Human UBE3A.

  • Positive control

    • WB: HeLa cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab3519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 100 kDa).

By Western blot, this antibody detects a single ~100 kDa protein representing E6-AP from HeLa cell lysate. IMPORTANT: E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots.

IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
  • Pathway

    Protein modification; protein ubiquitination.
  • Involvement in disease

    Angelman syndrome
  • Sequence similarities

    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
  • Post-translational
    modifications

    Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
  • Cellular localization

    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANCR antibody
    • Angelman syndrome antibody
    • AS antibody
    • CTCL tumor antigen se37 2 antibody
    • CTCL tumor antigen se37-2 antibody
    • E6 AP antibody
    • E6AP antibody
    • E6AP ubiquitin protein ligase antibody
    • E6AP ubiquitin-protein ligase antibody
    • EPVE6AP antibody
    • FLJ26981 antibody
    • HECT-type ubiquitin transferase E3A antibody
    • HPVE6A antibody
    • Human papilloma virus E6 associated protein Angelman syndrome antibody
    • Human papilloma virus E6 associated protein antibody
    • Human papillomavirus E6-associated protein antibody
    • NY REN 54 antigen antibody
    • Oncogenic protein associated protein E6 AP antibody
    • Oncogenic protein associated protein E6AP antibody
    • Oncogenic protein-associated protein E6-AP antibody
    • Renal carcinoma antigen NY REN 54 antibody
    • Renal carcinoma antigen NY-REN-54 antibody
    • UBE 3A antibody
    • Ube3a antibody
    • UBE3A protein antibody
    • UBE3A_HUMAN antibody
    • Ubiquitin protein ligase E3A antibody
    • Ubiquitin-protein ligase E3A antibody
    see all

Images

  • Western blot detection of UBE3A in HeLa cell extract using ab3519.

References

ab3519 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your email. I have checked with the originator of this antibody and it does seem that the protein that was used was most likely degraded (when E6-AP degrades a 50 kDa band seen in Western blots). However, we really don't know why the other antibody is working and this one is not. Would you be able to send a Western blot image as a jpg attachment so we can review it? It would be very helpful. Thanks again.

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Answer

Thank you for the details that you have provided. Please note that E6-AP is a very labile protein. Freeze/thaw cycling of samples results in E6-AP degradation which is observed as a prominent 50 kDa band seen in Western blots. This may explain why your customer is seeing a band at approximately 60 kDa. So we suggest running fresh samples, and in addition, the concentration of antibody and incubation period may need to be increased. Please let us know if you need additional assistance.

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