Overview

  • Product name

    Anti-UBE3A antibody [EPR7330]
    See all UBE3A primary antibodies
  • Description

    Rabbit monoclonal [EPR7330] to UBE3A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human UBE3A (Q05086).

  • Positive control

    • Jurkat, HeLa, and K562 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 100 kDa.

For unpurified use at 1/1000 - 1/1000.

  • Application notes
    Is unsuitable for ICC or IHC-P.
  • Target

    • Function

      E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B. Catalyzes the high-risk human papilloma virus E6-mediated ubiquitination of p53/TP53, contributing to the neoplastic progression of cells infected by these viruses. Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo. Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990).
    • Pathway

      Protein modification; protein ubiquitination.
    • Involvement in disease

      Angelman syndrome
    • Sequence similarities

      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    • Post-translational
      modifications

      Phosphorylation at Tyr-659 by ABL1 impairs E3 ligase activity and protects p53/TP53 from degradation in (HPV)-infected cells.
    • Cellular localization

      Nucleus. Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • ANCR antibody
      • Angelman syndrome antibody
      • AS antibody
      • CTCL tumor antigen se37 2 antibody
      • CTCL tumor antigen se37-2 antibody
      • E6 AP antibody
      • E6AP antibody
      • E6AP ubiquitin protein ligase antibody
      • E6AP ubiquitin-protein ligase antibody
      • EPVE6AP antibody
      • FLJ26981 antibody
      • HECT-type ubiquitin transferase E3A antibody
      • HPVE6A antibody
      • Human papilloma virus E6 associated protein Angelman syndrome antibody
      • Human papilloma virus E6 associated protein antibody
      • Human papillomavirus E6-associated protein antibody
      • NY REN 54 antigen antibody
      • Oncogenic protein associated protein E6 AP antibody
      • Oncogenic protein associated protein E6AP antibody
      • Oncogenic protein-associated protein E6-AP antibody
      • Renal carcinoma antigen NY REN 54 antibody
      • Renal carcinoma antigen NY-REN-54 antibody
      • UBE 3A antibody
      • Ube3a antibody
      • UBE3A protein antibody
      • UBE3A_HUMAN antibody
      • Ubiquitin protein ligase E3A antibody
      • Ubiquitin-protein ligase E3A antibody
      see all

    Images

    • All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/10000 dilution

      Lane 1 : Mouse brain lysate
      Lane 2 : Rat brain lysate
      Lane 3 : Rat spleen lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 100 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : 293 cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), HRP-conjugated at 1/1000 dilution

      Predicted band size: 100 kDa



      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-UBE3A antibody [EPR7330] (ab126765) at 1/1000 dilution (unpuified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 100 kDa

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Urraca N  et al. Significant transcriptional changes in 15q duplication but not Angelman syndrome deletion stem cell-derived neurons. Mol Autism 9:6 (2018). WB . Read more (PubMed: 29423132) »
    • Wu S & Chen H Anti-Condyloma acuminata mechanism of microRNAs-375 modulates HPV in cervical cancer cells via the UBE3A and IGF-1R pathway. Oncol Lett 16:3241-3247 (2018). Read more (PubMed: 30127920) »
    See all 3 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (HeLa and SiHa cells)
    Total protein in input
    1000 µg
    Immuno-precipitation step
    Protein G
    Specification
    HeLa and SiHa cells

    Abcam user community

    Verified customer

    Submitted Apr 19 2018

    Answer

    Thank you for contacting us.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Answer

    Thank you for your response and interest in our antibodies!

    I'm happy to hear you found anti-ADH and anti-UBE3A antibodies that have been shown to work in ELISA, and I sincerely hope they work for you.

    Unfortunately, the Abtrial program applies prior to purchase rather than post-purchase and post-experiment. However, we welcome your submission of an Abreview for both ab102083 and ab126765, and you can collect Abpoints which you may redeem for products or Amazon gift cards while sharing information about the product with your colleagues worldwide. To leave an Abreview, go to the webpage for the product, click on the Abreview Tab right below the shipping information and click https://www.abcam.com/index.html?pageconfig=your_reviews&intAbID=106640&strAccessMode=public_create. You will gain more Abpoints by submitting images as well. More information may be at:

    https://www.abcam.com/abreviews

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Answer

    Thank you for contacting Abcam.

    Earlier we discussed on the phone that your antibodies (ab126765 and ab102083) you tested in ELISA did not produce a positive signal. Since both of these antibodies have not been tested in ELISA and are only guaranteed to work in western blot as stated on the datasheet, the abPromise guarantee does not apply in this case. As we discussed, if the antibodies are tested in western blot against a species indicated to be reactive on the datasheet you can contact us and if we cannot get them to work as stated on the datasheet we will issue a free of charge replacement or refund.

    We also discussed potential options for moving forward with your experiment. We do have an anti-ADH5 antibody that has been tested and guaranteed to work in ELISA against a human sample (ab91385, https://www.abcam.com/adh5-antibody-ab91385.html)

    Since we do not have an anti-UBE3A antibody that has been tested in ELISA, you could test one of them through our AbTrial program as we discussed. Further information about the AbTrial program is given below:

    By participating in our AbTrial program you can now use our products in an untested application or species without financial risk.

    Simply follow these easy steps below to apply for our AbTrial Program:

    1. Reply to this email, letting us know you are interested in testing this product.

    2. Our scientists will email you an inactive personal discount code for the value of the product.

    3. Purchase and test the product at the regular price.

    4. Submit your results, including your discount code in the additional notes section of your Abreview.

    5. Once the Abreview is submitted, the discount code will become active.

    6. Apply your discount code on your next order to receive that value off.

    The Terms and Conditions of this offer can be found at: https://www.abcam.com/abtrial

    If you are interested in the abtrial program, below is a list of our anti-UBE3A antibodies
    https://www.abcam.com/Search?AppliedFacets.FacetProductType=Primary+antibodies&IsFacetsOnly=False&PageNumber=1&Keywords[0]=ube3a&X-Requested-With=XMLHttpRequest

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up