Key features and details
- Mouse monoclonal [OTI3F10] to UBE3B
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2a
Product nameAnti-UBE3B antibody [OTI3F10]
See all UBE3B primary antibodies
DescriptionMouse monoclonal [OTI3F10] to UBE3B
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus tropicalis
Recombinant fragment corresponding to Human UBE3B aa 768-1068. Produced in E.coli (NP_569733).
HENYLQLFEFVGKMLGKAVYEGIVVDVPFASFFLSQLLGHHHSVFYSSVD ELPSLDSEFYKNLTSIKRYDGDITDLGLTLSYDEDVMGQLVCHELIPGGK TIPVTNENKISYIHLMAHFRMHTQIKNQTAALISGFRSIIKPEWIRMFST PELQRLISGDNAEIDLEDLKKHTVYYGGFHGSHRVIIWLWDILASDFTPD ERAMFLKFVTSCSRPPLLGFAYLKPPFSIRCVEVSDDQDTGDTLGSVLRG FFTIRKREPGGRLPTSSTCFNLLKLPNYSKKSVLREKLRYAISMNTGFEL S
Database link: Q7Z3V4
- IHC-P: Human breast adenocarcinoma, thyroid carcinoma, spleen and appendix tissues.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant.
Our Abpromise guarantee covers the use of ab236424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Perform heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
RelevanceUBE3B accepts ubiquitin from an E2 ubiquitin conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. UBE3B may interact with other proteins and play a role in stress response. Alternatively spliced transcript variants encoding the same protein isoform have been identified for this gene.
- AI449831 antibody
- AU020130 antibody
- FLJ45294 antibody
Paraffin-embedded human appendix tissue stained for UBE3B using ab236424 at 1/250 dilution in immunohistochemical analysis.
Paraffin-embedded human spleen tissue stained for UBE3B using ab236424 at 1/250 dilution in immunohistochemical analysis.
Heat-induced epitope retrieval was performed using 1 mM EDTA in 10 mM Tris buffer (pH 8.5) at 120°C for 3 minutes.
Paraffin-embedded human thyroid carcinoma tissue stained for UBE3B using ab236424 at 1/250 dilution in immunohistochemical analysis.
Paraffin-embedded human breast adenocarcinoma tissue stained for UBE3B using ab236424 at 1/250 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236424 has not yet been referenced specifically in any publications.