Anti-UBE3C antibody (ab177511)

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Immunoprecipitation - Anti-UBE3C antibody (ab177511)

    Key features and details

    • Rabbit polyclonal to UBE3C
    • Suitable for: IP
    • Reacts with: Human
    • Isotype: IgG

    Overview

    • Product name

      Anti-UBE3C antibody
      See all UBE3C primary antibodies

    • Description

      Rabbit polyclonal to UBE3C

    • Host species

      Rabbit

    • Tested applications

      Suitable for: IPmore details

    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Xenopus tropicalis

    • Immunogen

      Synthetic peptide within Human UBE3C aa 725-775. The exact sequence is proprietary. (NP_055486.2)
      Sequence:

      VTIRRN YIYEDAYDKL SPENEPDLKK RIRVHLLNAH GLDEAGIDGG GIFRE


      Database link: Q15386
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • Positive control

      • 293T whole cell lysate (ab95494) can be used as a positive control in WB.

    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    • Storage buffer

      pH: 7
      Preservative: 0.09% Sodium azide
      Constituent: 99% Tris citrate/phosphate

      pH 7 - 8
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Purification notes

      ab177511 was affinity purified using an epitope specific to UBE3C immobilized on solid support.

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    Our Abpromise guarantee covers the use of ab177511 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IP Use at 2-10 µg/mg of lysate.

    Target

    • Function

      E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo.

    • Tissue specificity

      Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 1 IQ domain.

    • Domain

      The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly.

    • Cellular localization

      Nucleus.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • HectH2 antibody
      • KIAA0010 antibody
      • KIAA10 antibody
      • Ube3c antibody
      • UBE3C_HUMAN antibody
      • Ubiquitin protein isopeptide ligase (E3) antibody
      • Ubiquitin protein ligase E3C antibody
      • Ubiquitin-protein ligase E3C antibody
      see all

    Images

    • Immunoprecipitation - Anti-UBE3C antibody (ab177511)
      Immunoprecipitation - Anti-UBE3C antibody (ab177511)

      Detection of Human UBE3C by Western Blot of Immunoprecipitates.

      Lane 1: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells.

      Lane 2: Control IgG.

      ab177511 used for IP at 6 μg/mg lysate. 

      Detection: Chemiluminescence with an exposure time of 3 seconds.

    References (0)

    Publishing research using ab177511? Please let us know so that we can cite the reference in this datasheet.

    ab177511 has not yet been referenced specifically in any publications.

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