Anti-UBE3C antibody (ab177511)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-UBE3C antibody
See all UBE3C primary antibodies -
Description
Rabbit polyclonal to UBE3C -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details
Unsuitable for: WB -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Xenopus tropicalis
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Immunogen
Synthetic peptide within Human UBE3C aa 725-775. The exact sequence is proprietary. (NP_055486.2)
Sequence:VTIRRN YIYEDAYDKL SPENEPDLKK RIRVHLLNAH GLDEAGIDGG GIFRE
Database link: Q15386 -
Positive control
- 293T whole cell lysate (ab95494) can be used as a positive control in WB.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 - 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab177511 was affinity purified using an epitope specific to UBE3C immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab177511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
E3 ubiquitin-protein ligase that accepts ubiquitin from the E2 ubiquitin-conjugating enzyme UBE2D1 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Can assemble unanchored poly-ubiquitin chains in either 'Lys-29'- or 'Lys-48'-linked polyubiquitin chains. Has preference for 'Lys-48' linkages. It can target itself for ubiquitination in vitro and may promote its own degradation in vivo. -
Tissue specificity
Highly expressed in skeletal muscle. Detected at much lower levels in kidney and pancreas. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 IQ domain. -
Domain
The C-terminal is necessary and sufficient for the poly-ubiquitin chain assembly. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 472637 Chimpanzee
- Entrez Gene: 508513 Cow
- Entrez Gene: 482822 Dog
- Entrez Gene: 9690 Human
- Entrez Gene: 100763 Mouse
- Entrez Gene: 100525843 Pig
- Entrez Gene: 362294 Rat
- Entrez Gene: 393445 Zebrafish
see all -
Alternative names
- HectH2 antibody
- KIAA0010 antibody
- KIAA10 antibody
see all
Images
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Immunoprecipitation - Anti-UBE3C antibody (ab177511)Detection of Human UBE3C by Western Blot of Immunoprecipitates.
Lane 1: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells.
Lane 2: Control IgG.
ab177511 used for IP at 6 μg/mg lysate.
Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
References
ab177511 has not yet been referenced specifically in any publications.
