Key features and details
- Rabbit polyclonal to UBE3D
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBE3D antibody
DescriptionRabbit polyclonal to UBE3D
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
- Human heart muscle tissue; RT 4, U 251 MG, Human liver and tonsil lysate.
This product was previously labelled as UBE2CBP
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab121927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
|WB||1/250 - 1/500.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionE3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome.
PathwayProtein modification; protein ubiquitination.
DomainThe C-terminal half (AA 188-389) is able to bind cyclin B and shows a self-ubiquitinylation activity (mono-, poly, or multi-ubiquitinylation) in a HECT-like sequence dependent manner.
modificationsUbiquitinated by UBCH10 (E2 ubiquitin-conjugating enzyme).
- Information by UniProt
- C6orf157 antibody
- chromosome 6 open reading frame 157 antibody
- DKFZp434A1520 antibody
Immunofluorescent staining of Human cell line U-2 OS shows positivity in mitochondria. Recommended concentration of ab121927 1-4 µg/ml. Cells treated with PFA/Triton X-100.
All lanes : Anti-UBE3D antibody (ab121927) at 1/250 dilution
Lane 1 : RT 4 cell line lysate
Lane 2 : U 251 MG cell line lysate
Lane 3 : Human plasma lysate
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
ab121927, at 1/20, staining UBE3D in paraffin embedded Human heart muscle tissue by Immunohistochemistry.
ab121927 has not yet been referenced specifically in any publications.