Overview

  • Product name

  • Description

    Rabbit polyclonal to Ube4a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Orangutan
  • Immunogen

    Recombinant fragment corresponding to Human Ube4a aa 653-742.
    Sequence:

    SLEHVLHFITIFTGSIERMKNPHLRAKLAEVLEAVMPHLDQTPNPLVSSV FHRKRVFCNFQYAPQLAEALIKVFVDIEFTGDPHQFEQKF


    Database link: Q14139

  • Positive control

    • RT-4 and U-251 MG cell lysates; MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab221377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

WB 1/100 - 1/250. Predicted molecular weight: 123 kDa.

Target

Images

  • Immunofluorescent analysis of PFA-fixed, Triton X-100 permeabilized MCF7 cells labeling Ube4a with ab221377 at 4 μg/ml (green).

  • All lanes : Anti-Ube4a antibody (ab221377) at 1/100 dilution

    Lane 1 : RT-4 cell lysate
    Lane 2 : U-251 MG cell lysate

    Predicted band size: 123 kDa

References

ab221377 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221377.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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