Key features and details
- Rabbit polyclonal to Ube4a
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Ube4a antibody
See all Ube4a primary antibodies
DescriptionRabbit polyclonal to Ube4a
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
Recombinant fragment corresponding to Human Ube4a aa 797-1066.
FLLDEAIQYLSKIKIQQIEKDRGEWDSLTPEARREKEAGLQMFGQLARFH NIMSNETIGTLAFLTSEIKSLFVHPFLAERIISMLNYFLQHLVGPKMGAL KVKDFSEFDFKPQQLVSDICTIYLNLGDEENFCATVPKDGRSYSPTLFAQ TVRVLKKINKPGNMIMAFSNLAERIKSLADLQQQEEETYADACDEFLDPI MSTLMCDPVVLPSSRVTVDRSTIARHLLSDQTDPFNRSPLTMDQIRPNTE LKEKIQRWLAERKQQKEQLE
Database link: Q14139
- WB: K562, MCF7, Jurkat, Caco-2, HepG2 and HEK-293T whole cell lysate. IHC-P: Human glioma and kidney tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230315 in the following tested applications.
|IHC-P||1/20 - 1/200.|
|WB||1/1000 - 1/5000. Detects a band of approximately 123 kDa (predicted molecular weight: 123, 124 kDa).|
FunctionBinds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
Sequence similaritiesBelongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain.
- Information by UniProt
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
All lanes : Anti-Ube4a antibody (ab230315) at 1/1000 dilution
Lane 1 : K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 2 : MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 6 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 123, 124 kDa
Observed band size: 123 kDa why is the actual band size different from the predicted?
Paraffin-embedded human glioma tissue stained for Ube4a with ab230315 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for Ube4a with ab230315 at 1/100 dilution in immunohistochemical analysis.
ab230315 has not yet been referenced specifically in any publications.