Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7331] to Ube4a - BSA and Azide free
- Suitable for: ICC, Flow Cyt, WB
- Reacts with: Human
Product nameAnti-Ube4a antibody [EPR7331] - BSA and Azide free
See all Ube4a primary antibodies
DescriptionRabbit monoclonal [EPR7331] to Ube4a - BSA and Azide free
Tested applicationsSuitable for: ICC, Flow Cyt, WBmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Ube4a aa 1-100. The exact sequence is proprietary.
Database link: Q14139
Ab248191 is the carrier-free version of ab128861. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab248191 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C. Do Not Freeze.
Dissociation constant (KD)KD = 1.91 x 10 -11 M Learn more about KD
Storage bufferpH: 7.2
Concentration information loading...
Our Abpromise guarantee covers the use of ab248191 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 125 kDa (predicted molecular weight: 123 kDa).|
FunctionBinds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
Sequence similaritiesBelongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain.
- Information by UniProt
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
All lanes : Anti-Ube4a antibody [EPR7331] (ab128861) at 1/1000 dilution
Lane 1 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 123 kDa
Observed band size: 125 kDa why is the actual band size different from the predicted?
This data was developed using ab128861, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab248191 has not yet been referenced specifically in any publications.