Recombinant Anti-Ube4a antibody [EPR7332] (ab137074)
![Western blot - Anti-Ube4a antibody [EPR7332] (ab137074)](https://www.abcam.com/ps/products/137/ab137074/Images/Ube4a-Primary-antibodies-ab137074-1.jpg "Western blot - Anti-Ube4a antibody [EPR7332] (ab137074)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7332] to Ube4a
- Suitable for: WB, ICC/IF
- Reacts with: Human
Overview
-
Product name
Anti-Ube4a antibody [EPR7332]
See all Ube4a primary antibodies -
Description
Rabbit monoclonal [EPR7332] to Ube4a -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic peptide within Human Ube4a aa 1050-1150 (C terminal). The exact sequence is proprietary.
-
Positive control
- 293T, K562, Caco-2 and HepG2 cell lysates; HepG2 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7332 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Human
|
| All applications |
Mouse
Rat
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
|
| ICC/IF | (1) |
1/100 - 1/250.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 123 kDa. |
|
ICC/IF
1/100 - 1/250. |
Target
-
Function
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. -
Sequence similarities
Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain. - Information by UniProt
-
Database links
- Entrez Gene: 9354 Human
- Entrez Gene: 140630 Mouse
- Entrez Gene: 315608 Rat
- Omim: 603753 Human
- SwissProt: Q14139 Human
- SwissProt: E9Q735 Mouse
- SwissProt: Q6P7A2 Rat
- Unigene: 602967 Human
see all -
Alternative names
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
see all
Images
-
Western blot - Anti-Ube4a antibody [EPR7332] (ab137074)All lanes : Anti-Ube4a antibody [EPR7332] (ab137074) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 123 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] (ab137074)](https://www.abcam.com/ps/products/137/ab137074/Images/Ube4a-Primary-antibodies-ab137074-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] (ab137074)Immunofluorescent analysis of HepG2 cells labelling Ube4a with ab137074 at 1/100 dilution. -
![Anti-Ube4a antibody [EPR7332] (ab137074)](https://www.abcam.com/ps/products/137/ab137074/Images/ab137074-3-benefits-of-recombinant-antibodies.png)
Anti-Ube4a antibody [EPR7332] (ab137074)
Protocols
Datasheets and documents
References (0)
ab137074 has not yet been referenced specifically in any publications.
