Recombinant Anti-Ube4a antibody [EPR7332] (ab137074)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Ube4a antibody [EPR7332]
See all Ube4a primary antibodies -
Description
Rabbit monoclonal [EPR7332] to Ube4a -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Ube4a aa 1050-1150 (C terminal). The exact sequence is proprietary.
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Positive control
- 293T, K562, Caco-2 and HepG2 cell lysates; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7332 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab137074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 123 kDa. | |
| ICC/IF | 1/100 - 1/250. | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. -
Sequence similarities
Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain. - Information by UniProt
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Database links
- Entrez Gene: 9354 Human
- Entrez Gene: 140630 Mouse
- Entrez Gene: 315608 Rat
- Omim: 603753 Human
- SwissProt: Q14139 Human
- SwissProt: E9Q735 Mouse
- SwissProt: Q6P7A2 Rat
- Unigene: 602967 Human
see all -
Alternative names
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
see all
Images
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![Western blot - Anti-Ube4a antibody [EPR7332] (ab137074)](https://www.abcam.com/ps/datasheet/images/137/ab137074/Ube4a-Primary-antibodies-ab137074-1.jpg)
Western blot - Anti-Ube4a antibody [EPR7332] (ab137074)All lanes : Anti-Ube4a antibody [EPR7332] (ab137074) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 123 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] (ab137074)](https://www.abcam.com/ps/datasheet/images/137/ab137074/Ube4a-Primary-antibodies-ab137074-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] (ab137074)Immunofluorescent analysis of HepG2 cells labelling Ube4a with ab137074 at 1/100 dilution.
Protocols
Datasheets and documents
References
ab137074 has not yet been referenced specifically in any publications.
