Product nameAnti-Ube4a antibody [EPR7332]
See all Ube4a primary antibodies
DescriptionRabbit monoclonal [EPR7332] to Ube4a
Tested applicationsSuitable for: WB, ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Ube4a aa 1050-1150 (C terminal). The exact sequence is proprietary.
- 293T, K562, Caco-2 and HepG2 cell lysates; HepG2 cells.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab137074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 123 kDa.|
|ICC/IF||1/100 - 1/250.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionBinds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
Sequence similaritiesBelongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain.
- Information by UniProt
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
All lanes : Anti-Ube4a antibody [EPR7332] (ab137074) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Caco-2 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 123 kDa
Immunofluorescent analysis of HepG2 cells labelling Ube4a with ab137074 at 1/100 dilution.
ab137074 has not yet been referenced specifically in any publications.