Overview

  • Product name

    Anti-Ube4a antibody [EPR7332]
    See all Ube4a primary antibodies
  • Description

    Rabbit monoclonal [EPR7332] to Ube4a
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ube4a aa 1050-1150 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, K562, Caco-2 and HepG2 cell lysates; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab137074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF 1/100 - 1/250.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    Images

    • All lanes : Anti-Ube4a antibody [EPR7332] (ab137074) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Caco-2 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 123 kDa

    • Immunofluorescent analysis of HepG2 cells labelling Ube4a with ab137074 at 1/100 dilution.

    References

    ab137074 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Specification
    U2OS
    Permeabilization
    No
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 25 2014

    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Treatment
    siRNA against UBE4A

    Abcam user community

    Verified customer

    Submitted Feb 21 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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