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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Ubiquitylation
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RecombinantRabMAb

Recombinant Anti-Ube4a antibody [EPR7332] (ab137074)

  • Datasheet
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Western blot - Anti-Ube4a antibody [EPR7332] (ab137074)
  • Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] (ab137074)
  • Anti-Ube4a antibody [EPR7332] (ab137074)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7332] to Ube4a
  • Suitable for: WB, ICC/IF
  • Reacts with: Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-Ube4a antibody [EPR7332]
    See all Ube4a primary antibodies
  • Description

    Rabbit monoclonal [EPR7332] to Ube4a
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Ube4a aa 1050-1150 (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, K562, Caco-2 and HepG2 cell lysates; HepG2 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7332
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Ubiquitylation
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Other E3 Ligases

Associated products

  • Alternative Versions

    • Anti-Ube4a antibody [EPR7332] - BSA and Azide free (ab248800)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Caco-2 whole cell lysate (ab3950)
    • K-562 whole cell lysate (ab7911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137074 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF (1)
1/100 - 1/250.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 123 kDa.
ICC/IF
1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
    • Sequence similarities

      Belongs to the ubiquitin conjugation factor E4 family.
      Contains 1 U-box domain.
    • Target information above from: UniProt accession Q14139 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9354 Human
      • Entrez Gene: 140630 Mouse
      • Entrez Gene: 315608 Rat
      • Omim: 603753 Human
      • SwissProt: Q14139 Human
      • SwissProt: E9Q735 Mouse
      • SwissProt: Q6P7A2 Rat
      • Unigene: 602967 Human
      • Unigene: 75275 Human
      • Unigene: 215069 Mouse
      • Unigene: 102204 Rat
      see all
    • Alternative names

      • E4 antibody
      • KIAA0126 antibody
      • MGC133315 antibody
      • UBE4A antibody
      • UBE4A_HUMAN antibody
      • Ubiquitin conjugation factor E4 A antibody
      • ubiquitination factor E4A (UFD2 homolog yeast) antibody
      • Ubiquitination factor E4A antibody
      • UBOX2 antibody
      • UFD2 antibody
      • UFD2 homolog antibody
      • UFD2b antibody
      see all

    Images

    • Western blot - Anti-Ube4a antibody [EPR7332] (ab137074)
      Western blot - Anti-Ube4a antibody [EPR7332] (ab137074)
      All lanes : Anti-Ube4a antibody [EPR7332] (ab137074) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : Caco-2 cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 123 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] (ab137074)
      Immunocytochemistry/ Immunofluorescence - Anti-Ube4a antibody [EPR7332] (ab137074)
      Immunofluorescent analysis of HepG2 cells labelling Ube4a with ab137074 at 1/100 dilution.
    • Anti-Ube4a antibody [EPR7332] (ab137074)
      Anti-Ube4a antibody [EPR7332] (ab137074)

    Protocols

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab137074? Please let us know so that we can cite the reference in this datasheet.

    ab137074 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Ube4a antibody [EPR7332]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (U2OS)
    Specification
    U2OS
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 25 2014

    Western blot abreview for Anti-Ube4a antibody [EPR7332]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (8%)
    Sample
    Human Cell lysate - whole cell (U2OS)
    Specification
    U2OS
    Treatment
    siRNA against UBE4A
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 21 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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