Product nameAnti-Ube4a antibody - N-terminal
See all Ube4a primary antibodies
DescriptionRabbit polyclonal to Ube4a - N-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to Human Ube4a (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q14139
- IHC-P: Human spleen and brain cortex tissue. WB: HepG2, SP2/0 and H9c2 whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/500 - 1/1000. Predicted molecular weight: 123 kDa.|
FunctionBinds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
Sequence similaritiesBelongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain.
- Information by UniProt
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
All lanes : Anti-Ube4a antibody - N-terminal (ab230661) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : SP2/0 whole cell lysate
Lane 3 : H9c2 (Rat heart myoblast cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 123 kDa
Formalin-fixed, paraffin-embedded human spleen tissue stained for Ube4a with ab230661 at a 1:100 dilution in immunohistochemical analysis.
Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Ube4a with ab230661 at a 1:100 dilution in immunohistochemical analysis.
ab230661 has not yet been referenced specifically in any publications.