Anti-Ube4a antibody - N-terminal (ab230661)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Ube4a antibody - N-terminal
See all Ube4a primary antibodies -
Description
Rabbit polyclonal to Ube4a - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Ube4a (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q14139 -
Positive control
- IHC-P: Human spleen and brain cortex tissue. WB: HepG2, SP2/0 and H9c2 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab230661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 123 kDa. |
Target
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Function
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. -
Sequence similarities
Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain. - Information by UniProt
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Database links
- Entrez Gene: 9354 Human
- Entrez Gene: 140630 Mouse
- Entrez Gene: 315608 Rat
- Omim: 603753 Human
- SwissProt: Q14139 Human
- SwissProt: E9Q735 Mouse
- SwissProt: Q6P7A2 Rat
- Unigene: 602967 Human
see all -
Alternative names
- E4 antibody
- KIAA0126 antibody
- MGC133315 antibody
see all
Images
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Western blot - Anti-Ube4a antibody - N-terminal (ab230661)All lanes : Anti-Ube4a antibody - N-terminal (ab230661) at 1/500 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : SP2/0 whole cell lysate
Lane 3 : H9c2 (Rat heart myoblast cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 123 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube4a antibody - N-terminal (ab230661)Formalin-fixed, paraffin-embedded human spleen tissue stained for Ube4a with ab230661 at a 1:100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ube4a antibody - N-terminal (ab230661)Formalin-fixed, paraffin-embedded human brain cortex tissue stained for Ube4a with ab230661 at a 1:100 dilution in immunohistochemical analysis.
Datasheets and documents
References
ab230661 has not yet been referenced specifically in any publications.
