Overview

  • Product name

  • Description

    Rabbit polyclonal to UBE4B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow, Zebrafish
  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 740-1020 of Human UBE4B (NP_006039).

  • Positive control

    • HepG2 whole cell lysate (ab7900); CA922 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: Isoform 2, 133 ; Isoform 1, 146 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
  • Tissue specificity

    Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
  • Sequence similarities

    Belongs to the ubiquitin conjugation factor E4 family.
    Contains 1 U-box domain.
  • Post-translational
    modifications

    Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • 4930551I19Rik antibody
    • 4933406G05Rik antibody
    • AU014668 antibody
    • D4Bwg0973e antibody
    • E4 antibody
    • HDNB1 antibody
    • Homozygously deleted in neuroblastoma 1 antibody
    • KIAA0684 antibody
    • mKIAA0684 antibody
    • RP23 173D8.1 antibody
    • Ube4b antibody
    • UBE4B_HUMAN antibody
    • Ubiquitin conjugation factor E4 B antibody
    • Ubiquitin fusion degradation protein 2 antibody
    • UBOX3 antibody
    • UFD2 antibody
    • UFD2a antibody
    • Ufd2p antibody
    see all

Images

  • Anti-UBE4B antibody (ab97697) at 1/10000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: Isoform 2, 133 ; Isoform 1, 146 kDa



    5% SDS PAGE
  • Immunohistochemical analysis of UBE4B in paraffin-embedded CA922 xenograft, using ab97697 at 1/500 dilution.

References

This product has been referenced in:

  • Woodfield SE  et al. Neuroblastoma patient outcomes, tumor differentiation, and ERK activation are correlated with expression levels of the ubiquitin ligase UBE4B. Genes Cancer 7:13-26 (2016). WB, IHC . Read more (PubMed: 27014418) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Retina, Cryosections)
Antigen retrieval step
None
Permeabilization
Yes - 0,5% Triton-x-100
Specification
Retina, Cryosections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Lisa Janina Klotz

Verified customer

Submitted Mar 28 2018

Answer

Thank you for your message and reply. I am very pleased to hear the customer would like to accept our offer and test ab97697 in ICC-IF. SiRNA data will be particularly useful to us and other customers. DISCOUNT CODE: ##### Expiration date: ###### This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ICC-IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount. If you have any further questions, please do not hesitate to contact us. We look forward to receiving the Abreview and wish the customer luck with their research.

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