Anti-UBE4B antibody (ab97697)
Key features and details
- Rabbit polyclonal to UBE4B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBE4B antibody
See all UBE4B primary antibodies -
Description
Rabbit polyclonal to UBE4B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish
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Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 740-1020 of Human UBE4B (NP_006039).
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Positive control
- HepG2 whole cell lysate (ab7900); CA922 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab97697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: Isoform 2, 133 ; Isoform 1, 146 kDa. | |
| IHC-P | 1/100 - 1/500. |
Target
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Function
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. -
Tissue specificity
Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen. -
Sequence similarities
Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain. -
Post-translational
modificationsProteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 520833 Cow
- Entrez Gene: 10277 Human
- Entrez Gene: 63958 Mouse
- Entrez Gene: 170784 Zebrafish
- SwissProt: O95155 Human
- SwissProt: Q9ES00 Mouse
- Unigene: 593974 Human
- Unigene: 393991 Mouse
see all -
Alternative names
- 4930551I19Rik antibody
- 4933406G05Rik antibody
- AU014668 antibody
see all
Images
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Western blot - Anti-UBE4B antibody (ab97697)Anti-UBE4B antibody (ab97697) at 1/10000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: Isoform 2, 133 ; Isoform 1, 146 kDa
5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBE4B antibody (ab97697)Immunohistochemical analysis of UBE4B in paraffin-embedded CA922 xenograft, using ab97697 at 1/500 dilution.
Protocols
References (1)
ab97697 has been referenced in 1 publication.
- Woodfield SE et al. Neuroblastoma patient outcomes, tumor differentiation, and ERK activation are correlated with expression levels of the ubiquitin ligase UBE4B. Genes Cancer 7:13-26 (2016). WB, IHC . PubMed: 27014418
