Anti-UBE4B antibody (ab97697)
Key features and details
- Rabbit polyclonal to UBE4B
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBE4B antibody
See all UBE4B primary antibodies -
Description
Rabbit polyclonal to UBE4B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow, Zebrafish -
Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 740-1020 of Human UBE4B (NP_006039).
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Positive control
- HepG2 whole cell lysate (ab7900); CA922 xenograft; 293T, A431, H1299, HeLa, Molt-4 and Raji cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 133 kDa.
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IHC-P | (1) |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 133 kDa. |
IHC-P
1/100 - 1/500. |
Target
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Function
Binds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3. -
Tissue specificity
Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen. -
Sequence similarities
Belongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain. -
Post-translational
modificationsProteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 520833 Cow
- Entrez Gene: 10277 Human
- Entrez Gene: 63958 Mouse
- Entrez Gene: 170784 Zebrafish
- SwissProt: O95155 Human
- SwissProt: Q9ES00 Mouse
- Unigene: 593974 Human
- Unigene: 393991 Mouse
see all -
Alternative names
- 4930551I19Rik antibody
- 4933406G05Rik antibody
- AU014668 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab97697 has been referenced in 3 publications.
- Zhu L et al. A clinically compatible drug-screening platform based on organotypic cultures identifies vulnerabilities to prevent and treat brain metastasis. EMBO Mol Med 14:e14552 (2022). PubMed: 35174975
- Gireud-Goss M et al. The ubiquitin ligase UBE4B regulates amyloid precursor protein ubiquitination, endosomal trafficking, and amyloid β42 generation and secretion. Mol Cell Neurosci 108:103542 (2020). PubMed: 32841720
- Woodfield SE et al. Neuroblastoma patient outcomes, tumor differentiation, and ERK activation are correlated with expression levels of the ubiquitin ligase UBE4B. Genes Cancer 7:13-26 (2016). WB, IHC . PubMed: 27014418