Product nameAnti-UBE4B antibody [EPR7471]
See all UBE4B primary antibodies
DescriptionRabbit monoclonal [EPR7471] to UBE4B
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human UBE4B aa 1-100. The exact sequence is proprietary.
- 293T, Raji, SK OV 3, L929, L6, RAW264.7, PC12, NIH 3T3, HeLa, and K562 cell lysates.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab126759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 146 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionBinds to the ubiquitin moieties of preformed conjugates and catalyzes ubiquitin chain assembly in conjunction with E1, E2, and E3.
Tissue specificityHighest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
Sequence similaritiesBelongs to the ubiquitin conjugation factor E4 family.
Contains 1 U-box domain.
modificationsProteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
- Information by UniProt
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All lanes : Anti-UBE4B antibody [EPR7471] (ab126759) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Raji cell lysate
Lane 3 : SK OV 3 cell lysate
Lane 4 : L929 cell lysate
Lane 5 : L6 cell lysate
Lane 6 : RAW264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH 3T3 cell lysate
Lane 9 : HeLa cell lysate
Lane 10 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP-conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 146 kDa
Western blot analysis of Human 293NT whole cell lysate labeling UBE4B with ab126759 at 1/2000. A HRP-conjugated goat anti-rabbit IgG polyclonal (1/20000) was used as the secondary antibody.
siRNA agains UBE4B was used to show for antibody specificity.
Unspecific siRNA (siLUC) was used as a negative control.
This product has been referenced in:
- Baranes-Bachar K et al. The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair. Mol Cell 69:866-878.e7 (2018). Read more (PubMed: 29499138) »