Product nameAnti-UBF1 antibody [EP2741Y]
See all UBF1 primary antibodies
DescriptionRabbit monoclonal [EP2741Y] to UBF1
Tested applicationsSuitable for: WB, IHC-P, ICC, Flow Cytmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human UBF1 aa 1-100 (N terminal). The exact sequence is proprietary.
- HeLa and A431 cell lysates, human breast carcinoma
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab75781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC||1/100 - 1/250.|
|Flow Cyt||1/30 - 1/50.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRecognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
Sequence similaritiesContains 6 HMG box DNA-binding domains.
modificationsPhosphorylated and activated by PIK3CA.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
- 90 kDa nucleolus organizer region autoantigen antibody
- Autoantigen NOR-90 antibody
- NOR 90 antibody
All lanes : Anti-UBF1 antibody [EP2741Y] (ab75781) at 1/500 dilution
Lane 1 : (A) HeLa cell
Lane 2 : (B) A431 cell
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 89 kDa
Observed band size: 89,89 kDa
Overlay histogram showing A431 cells stained with ab75781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75781, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab75781 at 1/100-1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This product has been referenced in:
- Stixová L et al. UVA irradiation strengthened an interaction between UBF1/2 proteins and H4K20 di-/tri-methylation. Chromosome Res 27:41-55 (2019). Read more (PubMed: 30610403) »
- Tchurikov NA et al. Hot spots of DNA double-strand breaks in human rDNA units are produced in vivo. Sci Rep 6:25866 (2016). Read more (PubMed: 27160357) »