Overview

  • Product name

    Anti-UBF1 antibody [EP2741Y] - BSA and Azide free
    See all UBF1 primary antibodies
  • Description

    Rabbit monoclonal [EP2741Y] to UBF1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, IHC-P, WB, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human UBF1 aa 1-100 (N terminal). The exact sequence is proprietary.

  • General notes

    ab239866 is the carrier-free version of ab75781 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239866 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
    • Sequence similarities

      Contains 6 HMG box DNA-binding domains.
    • Post-translational
      modifications

      Phosphorylated and activated by PIK3CA.
    • Cellular localization

      Nucleus > nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 90 kDa nucleolus organizer region autoantigen antibody
      • Autoantigen NOR-90 antibody
      • NOR 90 antibody
      • Nucleolar transcription factor 1 antibody
      • UBF 1 antibody
      • UBF antibody
      • UBF-1 antibody
      • UBF1_HUMAN antibody
      • Ubtf antibody
      • Upstream binding factor 1 antibody
      • upstream binding transcription factor, RNA polymerase I antibody
      • Upstream-binding factor 1 antibody
      see all

    Images

    • Overlay histogram showing A431 cells stained with ab75781 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75781, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75781).

    • Immunohistochemical analysis of paraffin-embedded human breast carcinoma using ab75781 at 1/100-1/250 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75781).

    References

    ab239866 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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