Overview

  • Product name

    Anti-UBF1 (phospho S484) antibody [EPR2725(2)]
    See all UBF1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2725(2)] to UBF1 (phospho S484)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBF1 aa 450-550 (phospho S484). The exact sequence is proprietary.
    Database link: P17480

  • Positive control

    • Nocodazole treated HeLa cell lysate; Human colon and squamous cell carcinoma of cervix tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR2725(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 94-97 kDa (predicted molecular weight: 89 kDa).
IHC-P 1/100.

Target

  • Function

    Recognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
  • Sequence similarities

    Contains 6 HMG box DNA-binding domains.
  • Post-translational
    modifications

    Phosphorylated and activated by PIK3CA.
  • Cellular localization

    Nucleus > nucleolus.
  • Information by UniProt
  • Database links

  • Alternative names

    • 90 kDa nucleolus organizer region autoantigen antibody
    • Autoantigen NOR-90 antibody
    • NOR 90 antibody
    • Nucleolar transcription factor 1 antibody
    • UBF 1 antibody
    • UBF antibody
    • UBF-1 antibody
    • UBF1_HUMAN antibody
    • Ubtf antibody
    • Upstream binding factor 1 antibody
    • upstream binding transcription factor, RNA polymerase I antibody
    • Upstream-binding factor 1 antibody
    see all

Images

  • All lanes : Anti-UBF1 (phospho S484) antibody [EPR2725(2)] (ab182583) at 1/10000 dilution

    Lane 1 : Nocodazole treated HeLa cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 89 kDa

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling UBF1 with ab182583 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

  • Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling UBF1 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

This product has been referenced in:

  • Kotani T  et al. Consecutive bouts of electrical stimulation-induced contractions alter ribosome biogenesis in rat skeletal muscle. J Appl Physiol (1985) 126:1673-1680 (2019). Read more (PubMed: 30998122) »
  • Ye Q  et al. Therapeutic Targeting of RNA Polymerase I With the Small-Molecule CX-5461 for Prevention of Arterial Injury-Induced Neointimal Hyperplasia. Arterioscler Thromb Vasc Biol 37:476-484 (2017). Read more (PubMed: 28062495) »
See all 3 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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