Anti-UBFD1 antibody (ab240696)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-UBFD1 antibody -
Description
Rabbit polyclonal to UBFD1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human UBFD1 aa 150-200. The exact sequence is proprietary.
Database link: O14562 -
Positive control
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab240696 was affinity purified using an epitope specific to UBFD1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab240696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 33 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
May play a role as NF-kappa-B regulator. -
Sequence similarities
Contains 1 ubiquitin-like domain. - Information by UniProt
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Database links
- Entrez Gene: 56061 Human
- Entrez Gene: 28018 Mouse
- SwissProt: O14562 Human
- SwissProt: Q78JW9 Mouse
- Unigene: 3459 Human
- Unigene: 282122 Mouse
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Alternative names
- Ubfd1 antibody
- UBFD1_HUMAN antibody
- Ubiquitin domain-containing protein UBFD1 antibody
see all
Images
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Western blot - Anti-UBFD1 antibody (ab240696)All lanes : Anti-UBFD1 antibody (ab240696) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-UBFD1 antibody (ab240696)UBFD1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240696 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240696 at 1 µg/ml.
Lane 1: ab240696 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HeK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
ab240696 has not yet been referenced specifically in any publications.
