Product nameAnti-UBFD1 antibody
DescriptionRabbit polyclonal to UBFD1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human UBFD1 aa 150-200. The exact sequence is proprietary.
Database link: O14562
- WB: HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 whole cell lysates. IP: HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab240696 was affinity purified using an epitope specific to UBFD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab240696 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 33 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionMay play a role as NF-kappa-B regulator.
Sequence similaritiesContains 1 ubiquitin-like domain.
- Information by UniProt
- Ubfd1 antibody
- UBFD1_HUMAN antibody
- Ubiquitin domain-containing protein UBFD1 antibody
All lanes : Anti-UBFD1 antibody (ab240696) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 5 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 33 kDa
Exposure time: 30 seconds
UBFD1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240696 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240696 at 1 µg/ml.
Lane 1: ab240696 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HeK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
ab240696 has not yet been referenced specifically in any publications.