Anti-UBIAD1 antibody - C-terminal (ab191691)
Key features and details
- Rabbit polyclonal to UBIAD1 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-UBIAD1 antibody - C-terminal -
Description
Rabbit polyclonal to UBIAD1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human UBIAD1 (C terminal). Peptide corresponds to 15 amino acids near the carboxy terminus (NP_037451.1).
Database link: Q9Y5Z9 -
Positive control
- Mouse liver tissue and tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab191691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Prenyltransferase that mediates the formation of menaquinone-4 (MK-4), a vitamin K2 isoform present at high concentrations in the brain, kidney and pancreas. Mediates the conversion of phylloquinone (PK) into menaquinone-4 (MK-4), probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. -
Tissue specificity
Ubiquitously expressed. -
Pathway
Cofactor biosynthesis; menaquinone biosynthesis. -
Involvement in disease
Defects in UBIAD1 are the cause of crystalline corneal dystrophy of Schnyder (SCCD) [MIM:121800]. SCCD is a rare autosomal dominant disease characterized by progressive corneal opacification resulting from abnormal deposition of cholesterol and phospholipids. -
Sequence similarities
Belongs to the ubiA prenyltransferase family. -
Cellular localization
Endoplasmic reticulum membrane. Cytoplasm. Nucleus. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 29914 Human
- Entrez Gene: 71707 Mouse
- Entrez Gene: 313706 Rat
- Omim: 611632 Human
- SwissProt: Q9Y5Z9 Human
- SwissProt: Q9DC60 Mouse
- SwissProt: D3ZG27 Rat
- Unigene: 522933 Human
see all -
Alternative names
- RP4-796F18.1 antibody
- SCCD antibody
- TERE 1 antibody
see all
Images
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Western blot - Anti-UBIAD1 antibody - C-terminal (ab191691)All lanes : Anti-UBIAD1 antibody - C-terminal (ab191691) at 1 µg/ml
Lane 1 : mouse liver tissue lysate with out blocking peptide
Lane 2 : mouse liver tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBIAD1 antibody - C-terminal (ab191691)Immunohistochemical staining of mouse liver tissue, labeling UBIAD1 using ab191691 at 20 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBIAD1 antibody - C-terminal (ab191691)Immunohistochemical analysis of paraffin-embedded mouse liver tissue, labeling UBIAD1 using ab191691 at 5 μg/mL.
Protocols
Datasheets and documents
References (1)
ab191691 has been referenced in 1 publication.
- Yan B et al. Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II-induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase. Mol Med Rep 16:6910-6915 (2017). PubMed: 28901410
