Anti-UBIAD1 antibody - C-terminal (ab191691)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-UBIAD1 antibody - C-terminal -
Description
Rabbit polyclonal to UBIAD1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human UBIAD1 (C terminal). Peptide corresponds to 15 amino acids near the carboxy terminus (NP_037451.1).
Database link: Q9Y5Z9 -
Positive control
- Mouse liver tissue and tissue lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab191691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 5 - 20 µg/ml. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Prenyltransferase that mediates the formation of menaquinone-4 (MK-4), a vitamin K2 isoform present at high concentrations in the brain, kidney and pancreas. Mediates the conversion of phylloquinone (PK) into menaquinone-4 (MK-4), probably by cleaving the side chain of phylloquinone (PK) to release 2-methyl-1,4-naphthoquinone (menadione; K3) and then prenylating it with geranylgeranyl pyrophosphate (GGPP) to form MK-4. -
Tissue specificity
Ubiquitously expressed. -
Pathway
Cofactor biosynthesis; menaquinone biosynthesis. -
Involvement in disease
Defects in UBIAD1 are the cause of crystalline corneal dystrophy of Schnyder (SCCD) [MIM:121800]. SCCD is a rare autosomal dominant disease characterized by progressive corneal opacification resulting from abnormal deposition of cholesterol and phospholipids. -
Sequence similarities
Belongs to the ubiA prenyltransferase family. -
Cellular localization
Endoplasmic reticulum membrane. Cytoplasm. Nucleus. Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 29914 Human
- Entrez Gene: 71707 Mouse
- Entrez Gene: 313706 Rat
- Omim: 611632 Human
- SwissProt: Q9Y5Z9 Human
- SwissProt: Q9DC60 Mouse
- SwissProt: D3ZG27 Rat
- Unigene: 522933 Human
see all -
Alternative names
- RP4-796F18.1 antibody
- SCCD antibody
- TERE 1 antibody
see all
Images
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Western blot - Anti-UBIAD1 antibody - C-terminal (ab191691)All lanes : Anti-UBIAD1 antibody - C-terminal (ab191691) at 1 µg/ml
Lane 1 : mouse liver tissue lysate with out blocking peptide
Lane 2 : mouse liver tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBIAD1 antibody - C-terminal (ab191691)Immunohistochemical staining of mouse liver tissue, labeling UBIAD1 using ab191691 at 20 μg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UBIAD1 antibody - C-terminal (ab191691)Immunohistochemical analysis of paraffin-embedded mouse liver tissue, labeling UBIAD1 using ab191691 at 5 μg/mL.
Datasheets and documents
References
This product has been referenced in:
- Yan B et al. Depletion of ubiA prenyltransferase domain containing 1 expression promotes angiotensin II-induced hypertrophic response in AC16 human myocardial cells via modulating the expression levels of coenzyme Q10 and endothelial nitric oxide synthase. Mol Med Rep 16:6910-6915 (2017). Read more (PubMed: 28901410) »
