Overview

  • Product name
  • Description
    Rabbit polyclonal to Ubiquilin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Hamster, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse Ubiquilin aa 2-18.
    Sequence:

    AESAESGGPPGAQDSAA

  • Positive control
    • CHO cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab3341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract.

IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml. PubMed: 19112176

Target

  • Function
    Links CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors (By similarity). Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation.
  • Tissue specificity
    Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney.
  • Sequence similarities
    Contains 1 UBA domain.
    Contains 1 ubiquitin-like domain.
  • Domain
    Binds PSEN1 and PSEN2 via its UBA domain.
  • Post-translational
    modifications
    Phosphorylated.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DA41 antibody
    • DSK2 antibody
    • FLJ90054 antibody
    • hPLIC-1 antibody
    • hPLIC1 antibody
    • PLIC-1 antibody
    • PLIC1 antibody
    • Protein linking IAP with cytoskeleton 1 antibody
    • Ubiquilin-1 antibody
    • Ubiquilin1 antibody
    • UBQL1_HUMAN antibody
    • UBQLN1 antibody
    • UBQN antibody
    • XDRP1 antibody
    see all

Images

  • Western blot detection of Ubiquilin on CHO cell extract using ab3341.

References

This product has been referenced in:
  • Adegoke OO  et al. Overexpression of Ubiquilin-1 Alleviates Alzheimer's Disease-Caused Cognitive and Motor Deficits and Reduces Amyloid-ß Accumulation in Mice. J Alzheimers Dis 59:575-590 (2017). WB . Read more (PubMed: 28598849) »
  • Adusei DC  et al. Early developmental alterations in GABAergic protein expression in fragile X knockout mice. Neuropharmacology 59:167-71 (2010). WB ; Mouse . Read more (PubMed: 20470805) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Randal Tibbetts

Verified customer

Submitted May 23 2013

Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx for a new lot of ab3341.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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