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    ubiquilinubqln1-antibody-ab3341.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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Anti-Ubiquilin/UBQLN1 antibody (ab3341)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (5)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
  • Western blot - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)

Key features and details

  • Rabbit polyclonal to Ubiquilin/UBQLN1
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Human, Chinese hamster
  • Isotype: IgG

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Protein
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Recombinant Human Ubiquilin/UBQLN1 protein (ab153310)
Primary
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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Ubiquilin/UBQLN1 antibody
    See all Ubiquilin/UBQLN1 primary antibodies
  • Description

    Rabbit polyclonal to Ubiquilin/UBQLN1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Chinese hamster
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Mouse Ubiquilin/UBQLN1 aa 2-18.
    Sequence:

    AESAESGGPPGAQDSAA

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

     This product was previously labelled as Ubiquilin

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Ubiquilin/UBQLN1 protein (ab153310)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Chinese hamster
Application Abreviews Notes
IHC-P
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB (1)
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract.

Notes
IHC-P
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract.

Target

  • Function

    Links CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors (By similarity). Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation.
  • Tissue specificity

    Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney.
  • Sequence similarities

    Contains 1 UBA domain.
    Contains 1 ubiquitin-like domain.
  • Domain

    Binds PSEN1 and PSEN2 via its UBA domain.
  • Post-translational
    modifications

    Phosphorylated.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession Q9UMX0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29979 Human
    • Entrez Gene: 56085 Mouse
    • Omim: 605046 Human
    • SwissProt: Q9UMX0 Human
    • SwissProt: Q8R317 Mouse
    • Unigene: 9589 Human
    • Unigene: 182053 Mouse
    • Alternative names

      • DA41 antibody
      • DSK2 antibody
      • FLJ90054 antibody
      • hPLIC-1 antibody
      • hPLIC1 antibody
      • PLIC-1 antibody
      • PLIC1 antibody
      • Protein linking IAP with cytoskeleton 1 antibody
      • Ubiquilin-1 antibody
      • Ubiquilin1 antibody
      • UBQL1_HUMAN antibody
      • UBQLN1 antibody
      • UBQN antibody
      • XDRP1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)

      Immunohistochemistry analysis of Ubiquilin showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)

      Immunohistochemistry analysis of Ubiquilin showing staining in the cytoplasm and nucleus of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Western blot - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
      Western blot - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
      Anti-Ubiquilin/UBQLN1 antibody (ab3341) + CHO (Chinese hamster ovary cell line) whole cell lysate

      Predicted band size: 63 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)

      Immunohistochemistry analysis of Ubiquilin showing staining in the cytoplasm and nucleus of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (5)

    Publishing research using ab3341? Please let us know so that we can cite the reference in this datasheet.

    ab3341 has been referenced in 5 publications.

    • Hu C  et al. Inadequate ubiquitination-proteasome coupling contributes to myocardial ischemia-reperfusion injury. J Clin Invest 128:5294-5306 (2018). PubMed: 30204128
    • Adegoke OO  et al. Overexpression of Ubiquilin-1 Alleviates Alzheimer's Disease-Caused Cognitive and Motor Deficits and Reduces Amyloid-ß Accumulation in Mice. J Alzheimers Dis 59:575-590 (2017). WB . PubMed: 28598849
    • González-Pérez P  et al. Association of UBQLN1 mutation with Brown-Vialetto-Van Laere syndrome but not typical ALS. Neurobiol Dis 48:391-8 (2012). PubMed: 22766032
    • Adusei DC  et al. Early developmental alterations in GABAergic protein expression in fragile X knockout mice. Neuropharmacology 59:167-71 (2010). WB ; Mouse . PubMed: 20470805
    • Hiltunen M  et al. Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion. J Biol Chem 281:32240-53 (2006). ICC/IF ; Mouse . PubMed: 16945923

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Ubiquilin/UBQLN1 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    50 µg
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Dr. Randal Tibbetts

    Verified customer

    Submitted May 23 2013

    Question

    used same Ab for 2 years
    lot 925125 worked
    lot GR27748-2 did not work
    mouse cell lysate
    lots of bands: 66 kDa and many more bands
    same samples
    Ab: 1/1000 o.n 4 degree
    block: 5% milk
    2nd: OK

    Read More

    Abcam community

    Verified customer

    Asked on May 03 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx for a new lot of ab3341.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on May 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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