Anti-Ubiquilin/UBQLN1 antibody (ab3341)
Key features and details
- Rabbit polyclonal to Ubiquilin/UBQLN1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Chinese hamster
- Isotype: IgG
Overview
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Product name
Anti-Ubiquilin/UBQLN1 antibody
See all Ubiquilin/UBQLN1 primary antibodies -
Description
Rabbit polyclonal to Ubiquilin/UBQLN1 -
Host species
Rabbit -
Tested Applications & Species
Application Species IHC-P HumanWB Chinese hamster -
Immunogen
Synthetic peptide corresponding to Mouse Ubiquilin/UBQLN1 aa 2-18.
Sequence:AESAESGGPPGAQDSAA
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General notes
This product was previously labelled as Ubiquilin
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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IHC-P |
Human
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WB |
Chinese hamster
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Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.
By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract. |
Notes |
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IHC-P
1/20 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa. By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract. |
Target
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Function
Links CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors (By similarity). Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation. -
Tissue specificity
Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney. -
Sequence similarities
Contains 1 UBA domain.
Contains 1 ubiquitin-like domain. -
Domain
Binds PSEN1 and PSEN2 via its UBA domain. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 29979 Human
- Entrez Gene: 56085 Mouse
- Omim: 605046 Human
- SwissProt: Q9UMX0 Human
- SwissProt: Q8R317 Mouse
- Unigene: 9589 Human
- Unigene: 182053 Mouse
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Alternative names
- DA41 antibody
- DSK2 antibody
- FLJ90054 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
Immunohistochemistry analysis of Ubiquilin showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
Immunohistochemistry analysis of Ubiquilin showing staining in the cytoplasm and nucleus of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
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Anti-Ubiquilin/UBQLN1 antibody (ab3341) + CHO (Chinese hamster ovary cell line) whole cell lysate
Predicted band size: 63 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
Immunohistochemistry analysis of Ubiquilin showing staining in the cytoplasm and nucleus of paraffin-embedded mouse kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with ab3341 diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab3341 has been referenced in 5 publications.
- Hu C et al. Inadequate ubiquitination-proteasome coupling contributes to myocardial ischemia-reperfusion injury. J Clin Invest 128:5294-5306 (2018). PubMed: 30204128
- Adegoke OO et al. Overexpression of Ubiquilin-1 Alleviates Alzheimer's Disease-Caused Cognitive and Motor Deficits and Reduces Amyloid-ß Accumulation in Mice. J Alzheimers Dis 59:575-590 (2017). WB . PubMed: 28598849
- González-Pérez P et al. Association of UBQLN1 mutation with Brown-Vialetto-Van Laere syndrome but not typical ALS. Neurobiol Dis 48:391-8 (2012). PubMed: 22766032
- Adusei DC et al. Early developmental alterations in GABAergic protein expression in fragile X knockout mice. Neuropharmacology 59:167-71 (2010). WB ; Mouse . PubMed: 20470805
- Hiltunen M et al. Ubiquilin 1 modulates amyloid precursor protein trafficking and Abeta secretion. J Biol Chem 281:32240-53 (2006). ICC/IF ; Mouse . PubMed: 16945923