Anti-Ubiquilin/UBQLN1 antibody (ab3341)

Reviews (1)Q&A (1)References (5)

Overview

  • Product name

    Anti-Ubiquilin/UBQLN1 antibody
    See all Ubiquilin/UBQLN1 primary antibodies

  • Description

    Rabbit polyclonal to Ubiquilin/UBQLN1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Hamster, Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide corresponding to Mouse Ubiquilin/UBQLN1 aa 2-18.
    Sequence:

    AESAESGGPPGAQDSAA

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • CHO cell extract.

  • General notes

     This product was previously labelled as Ubiquilin

     

Properties

Applications

Our Abpromise guarantee covers the use of ab3341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.

By Western blot, this antibody detects an ~63 kDa protein representing Ubiquilin from CHO cell extract.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml. PubMed: 19112176

Target

  • Function

    Links CD47 to the cytoskeleton. Promotes the surface expression of GABA-A receptors (By similarity). Promotes the accumulation of uncleaved PSEN1 and PSEN2 by stimulating their biosynthesis. Has no effect on PSEN1 and PSEN2 degradation.

  • Tissue specificity

    Ubiquitous. Highly expressed throughout the brain; detected in neurons and in neuropathological lesions, such as neurofibrillary tangles and Lewy bodies. Highly expressed in heart, placenta, pancreas, lung, liver, skeletal muscle and kidney.

  • Sequence similarities

    Contains 1 UBA domain.
    Contains 1 ubiquitin-like domain.

  • Domain

    Binds PSEN1 and PSEN2 via its UBA domain.

  • Post-translational
    modifications

    Phosphorylated.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DA41 antibody
    • DSK2 antibody
    • FLJ90054 antibody
    • hPLIC-1 antibody
    • hPLIC1 antibody
    • PLIC-1 antibody
    • PLIC1 antibody
    • Protein linking IAP with cytoskeleton 1 antibody
    • Ubiquilin-1 antibody
    • Ubiquilin1 antibody
    • UBQL1_HUMAN antibody
    • UBQLN1 antibody
    • UBQN antibody
    • XDRP1 antibody
    see all

Images

  • Western blot - Anti-Ubiquilin/UBQLN1 antibody (ab3341)
    Western blot - Anti-Ubiquilin/UBQLN1 antibody (ab3341)

    Western blot detection of Ubiquilin on CHO cell extract using ab3341.

References

This product has been referenced in:

  • Hu C  et al. Inadequate ubiquitination-proteasome coupling contributes to myocardial ischemia-reperfusion injury. J Clin Invest 128:5294-5306 (2018). Read more (PubMed: 30204128) »
  • Adegoke OO  et al. Overexpression of Ubiquilin-1 Alleviates Alzheimer's Disease-Caused Cognitive and Motor Deficits and Reduces Amyloid-ß Accumulation in Mice. J Alzheimers Dis 59:575-590 (2017). WB . Read more (PubMed: 28598849) »
See all 5 Publications for this product

Publishing research using ab3341? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Ubiquilin/UBQLN1 antibody

 Good
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
U2OS
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Dr. Randal Tibbetts

Verified customer

Submitted May 23 2013

Question

used same Ab for 2 years
lot 925125 worked
lot GR27748-2 did not work
mouse cell lysate
lots of bands: 66 kDa and many more bands
same samples
Ab: 1/1000 o.n 4 degree
block: 5% milk
2nd: OK

Read More
Answer

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxx for a new lot of ab3341.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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