Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (HRP) (ab197968)

Overview

  • Product name

    Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (HRP)
    See all Ubiquinol-Cytochrome C Reductase Core Protein I primary antibodies
  • Description

    Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I (HRP)
  • Host species

    Mouse
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Full length native protein (purified) corresponding to Cow Ubiquinol-Cytochrome C Reductase Core Protein I.

  • Positive control

    • WB: Human heart mitochondrial lysate and HepG2 whole cell lysate. IHC-P: Normal human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab197968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 49 kDa (predicted molecular weight: 53 kDa).
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (HRP) (ab197968) at 1/5000 dilution + Human heart tissue lysate - mitochondrial extract (ab110337) at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?


    Exposure time: 18 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197968 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • Anti-Ubiquinol-Cytochrome C Reductase Core Protein I antibody [16D10AD9AH5] (HRP) (ab197968) at 1/5000 dilution + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 20 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 49 kDa why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab197968 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

  • IHC image of Ubiquinol-Cytochrome C Reductase Core Protein I staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab197968, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

ab197968 has not yet been referenced specifically in any publications.

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