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Anti-Ubiquitin antibody [1B4-UB] (ab122)

  • Datasheet
  • SDS
Reviews (1)Q&A (2)References (3)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody [1B4-UB] (ab122)

    Key features and details

    • Mouse monoclonal [1B4-UB] to Ubiquitin
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG1

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    Overview

    • Product name

      Anti-Ubiquitin antibody [1B4-UB]
      See all Ubiquitin primary antibodies
    • Description

      Mouse monoclonal [1B4-UB] to Ubiquitin
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin

    • Positive control

      • Human hypophysis

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Preservative: 0.1% Sodium azide
    • Concentration information loading...
    • Purity

      Protein G purified
    • Purification notes

      Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
    • Clonality

      Monoclonal
    • Clone number

      1B4-UB
    • Isotype

      IgG1
    • Light chain type

      unknown
    • Research areas

      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Alzheimer's disease
      • Tangles & Tau
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Ubiquitylation
      • Cell Biology
      • Proteolysis / Ubiquitin
      • Proteasome / Ubiquitin
      • Ubiquitin
      • Neuroscience
      • Neurology process
      • Neurodegenerative disease
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Recombinant Protein

      • Recombinant Human Ubiquitin protein (His tag) (ab218616)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab122 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    1/500 - 1/1000.
    Notes
    IHC-P
    1/500 - 1/1000.

    Target

    • Relevance

      Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localization

      Cell Membrane, Cytoplasmic and Nuclear
    • Database links

      • Entrez Gene: 7314 Human
      • SwissProt: P0CG47 Human
      • SwissProt: P0CG48 Human
      • SwissProt: P62979 Human
      • SwissProt: P62987 Human
      • SwissProt: P62988 Human
      • Unigene: 356190 Human
      • Unigene: 520348 Human
      • Alternative names

        • Epididymis secretory protein Li 50 antibody
        • FLJ25987 antibody
        • HEL S 50 antibody
        • MGC8385 antibody
        • Polyubiquitin B antibody
        • RPS 27A antibody
        • RPS27A antibody
        • UBA 52 antibody
        • UBA 80 antibody
        • UBA52 antibody
        • UBA80 antibody
        • UBB antibody
        • UBB_HUMAN antibody
        • UBC antibody
        • UBCEP 1 antibody
        • UBCEP 2 antibody
        • UBCEP1 antibody
        • UBCEP2 antibody
        • Ubiquitin antibody
        • Ubiquitin B antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody [1B4-UB] (ab122)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ubiquitin antibody [1B4-UB] (ab122)
        ab122 staining Ubiquitin in the tissue of human hypophysis by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab122? Please let us know so that we can cite the reference in this datasheet.

      ab122 has been referenced in 3 publications.

      • Pirooznia SK  et al. PARIS induced defects in mitochondrial biogenesis drive dopamine neuron loss under conditions of parkin or PINK1 deficiency. Mol Neurodegener 15:17 (2020). PubMed: 32138754
      • Shevade S  et al. Food vacuole associated enolase in plasmodium undergoes multiple post-translational modifications: evidence for atypical ubiquitination. PLoS One 8:e72687 (2013). PubMed: 24009698
      • Gao Z  et al. Sirtuin 1 (SIRT1) Protein Degradation in Response to Persistent c-Jun N-terminal Kinase 1 (JNK1) Activation Contributes to Hepatic Steatosis in Obesity. J Biol Chem 286:22227-34 (2011). WB ; Human . PubMed: 21540183

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-3 of 3 Abreviews or Q&A

      Western blot abreview for Anti-Ubiquitin antibody [1B4-UB]

      Good
      Abreviews
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Breast cancer Cells)
      Loading amount
      50 µg
      Specification
      Breast cancer Cells
      Gel Running Conditions
      Reduced Denaturing (4-12%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 12 2008

      Question

      Hi What is the epitope the antibody is raised against and do you know weather this antibody would recognise yeast ubiquitin

      Read More

      Abcam community

      Verified customer

      Asked on Jun 29 2001

      Answer

      This is a relatively new antibody that has not yet been epitope mapped or tried in yeast. We are about to implement a trial 'try risk free' scheme which says that if an antibody is purchased and used in an experiment outside that written on the datasheet, and it is found not to work, in return for the negative data (which we can add to the datasheet to help other researchers) we will replace the antibody for one of equivalent value or refund it. We hope this will help It is therefore risk free for the researcher and it helps build information into our datasheets.

      Read More

      Abcam Scientific Support

      Answered on Jul 02 2001

      Question

      Hi, how specific is this monoclonal human ubiquitin antibody in western blots? is it possible to see a picture of a western blot with this antibody? and does it only recognize ubiquitin itself or other polyubiquitinated species as well? Thanks

      Read More

      Abcam community

      Verified customer

      Asked on Jun 13 2001

      Answer

      A single band at 32kD is detected in Western blotting. Although we do have pictures of Western blots, these are of very poor quality and therefore of little help.

      Read More

      Neil Hayward

      Abcam Scientific Support

      Answered on Jun 20 2001

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