Overview

  • Product name
    Anti-Ubiquitin antibody [1B4-UB]
    See all Ubiquitin primary antibodies
  • Description
    Mouse monoclonal [1B4-UB] to Ubiquitin
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, WB, IHC-FoFr, ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    Ubiquitin from bovine erythrocytes conjugated to bovine gamma globulin

  • Positive control
    • Human hypophysis

Properties

Applications

Our Abpromise guarantee covers the use of ab122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.
IHC-Fr 1/500 - 1/1000.
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
Flow Cyt 1/10 - 1/50.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • Cellular localization
    Cell Membrane, Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Epididymis secretory protein Li 50 antibody
    • FLJ25987 antibody
    • HEL S 50 antibody
    • MGC8385 antibody
    • Polyubiquitin B antibody
    • RPS 27A antibody
    • RPS27A antibody
    • UBA 52 antibody
    • UBA 80 antibody
    • UBA52 antibody
    • UBA80 antibody
    • UBB antibody
    • UBB_HUMAN antibody
    • UBC antibody
    • UBCEP 1 antibody
    • UBCEP 2 antibody
    • UBCEP1 antibody
    • UBCEP2 antibody
    • Ubiquitin antibody
    • Ubiquitin B antibody
    see all

Images

  • ab122 staining Ubiquitin in the tissue of human hypophysis by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

This product has been referenced in:
  • Shevade S  et al. Food vacuole associated enolase in plasmodium undergoes multiple post-translational modifications: evidence for atypical ubiquitination. PLoS One 8:e72687 (2013). Read more (PubMed: 24009698) »
  • Gao Z  et al. Sirtuin 1 (SIRT1) Protein Degradation in Response to Persistent c-Jun N-terminal Kinase 1 (JNK1) Activation Contributes to Hepatic Steatosis in Obesity. J Biol Chem 286:22227-34 (2011). WB ; Human . Read more (PubMed: 21540183) »
See all 2 Publications for this product

Customer reviews and Q&As

Filter by Application

Filter by Species

Filter by Ratings

Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer Cells)
Loading amount
50 µg
Specification
Breast cancer Cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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Verified customer

Submitted Aug 12 2008

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