Western blot abreview for Anti-Ubiquitin antibody [1B4-UB]

Good
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer Cells)
Loading amount
50 µg
Specification
Breast cancer Cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Sheep anti mouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECl+
Exposure
30 second(s)
Bands
Specific: 8 kDa Non-specific: 16, 45, 53 kDa

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Submitted Aug 12 2008

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