Overview

  • Product name
  • Description
    Rabbit polyclonal to Ubiquitin
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Cow
  • Immunogen

    Full length native protein (purified) corresponding to Cow Ubiquitin conjugated to keyhole limpet haemocyanin.
    Database link: P0CG53

  • Positive control
    • WB: HEK-293 cell lysate. IHC-P: Mouse backskin tissue; Human colon carcinoma tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab223613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
WB 1/1000. Predicted molecular weight: 26 kDa.
IHC-P 1/100.

Target

  • Relevance
    Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • Cellular localization
    Cell Membrane, Cytoplasmic and Nuclear
  • Database links
  • Alternative names
    • Epididymis secretory protein Li 50 antibody
    • FLJ25987 antibody
    • HEL S 50 antibody
    • MGC8385 antibody
    • Polyubiquitin B antibody
    • RPS 27A antibody
    • RPS27A antibody
    • UBA 52 antibody
    • UBA 80 antibody
    • UBA52 antibody
    • UBA80 antibody
    • UBB antibody
    • UBB_HUMAN antibody
    • UBC antibody
    • UBCEP 1 antibody
    • UBCEP 2 antibody
    • UBCEP1 antibody
    • UBCEP2 antibody
    • Ubiquitin antibody
    • Ubiquitin B antibody
    see all

Images

  • 2% formaldehyde-fixed heat shocked (at 42°C for 1 hour) HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (yellow) using ab223613 at at 1/100 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit R-PE at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature. 

    A) DAPI (blue) nuclear stain. (B) ab223613. (C) Merge.

  • Anti-Ubiquitin antibody (ab223613) at 1/1000 dilution + HEK-293 (human epithelial cell line from embryonic kidney)cell lysate

    Predicted band size: 26 kDa

  • Mouse backskin tissue tissue stained for Ubiquitin (green) using ab223613 at 1/100 dilution in immunohistochemical analysis, followed by Goat Anti-Rabbit FITC at 1/50 dilution for 1 hour at room temperature.

    Fixation: Bouin’s Fixative Solution.

  • Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Ubiquitin using ab223613 at 1/100000 dilution in immunohistochemical analysis, followed by Goat Anti-Rabbit Biotin at 1/2000 dilution for 1 hour at room temperature.

  • 2% formaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Ubiquitin (green) using ab223613 at 1/200 dilution for 12 hours at 4°C in ICC/IF, followed by Goat Anti-Rabbit FITC at 1/200 for 2 hours at room temperature. Counterstain: DAPI (blue) nuclear stain at 1/40000 dilution for 2 hours at room temperature. 

    A) DAPI (blue) nuclear stain. (B) ab223613. (C) Merge.

References

ab223613 has not yet been referenced specifically in any publications.

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