• Product name
    Anti-Ubiquitin antibody [EP296Y]
    See all Ubiquitin primary antibodies
  • Description
    Rabbit monoclonal [EP296Y] to Ubiquitin
  • Host species
  • Specificity
    The antibody recognizes only mono-ubiquitinated molecules.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Ubiquitin aa 50-150 (C terminal). The exact sequence is proprietary.

  • Positive control
    • Hela cell lysate and human prostate adenocarcinoma tissue.
  • General notes

    A trial size is available to purchase for this antibody.


    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.



Our Abpromise guarantee covers the use of ab33893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 8.5 kDa (predicted molecular weight: 8 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Relevance
      Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localization
      Cell Membrane, Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Epididymis secretory protein Li 50 antibody
      • FLJ25987 antibody
      • HEL S 50 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all



    This product has been referenced in:
    • He Y  et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). Read more (PubMed: 27959342) »
    • Sidoryk-Wegrzynowicz M  et al. Disruption of astrocytic glutamine turnover by manganese is mediated by the protein kinase C pathway. Glia 59:1732-43 (2011). WB ; Human . Read more (PubMed: 21812036) »
    See all 6 Publications for this product

    Customer reviews and Q&As

    1-4 of 4 Abreviews or Q&A

    This product is known to not work in this application or species.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)
    Yes - 0.5% Triton X100 in PBS

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Mar 27 2014


    All of the information we have for each of our products is listed on the online datasheet for your convenience. These are updated as soon as any new information is brought to our attention.

    As ICC/IF is not listed as a tested application, we do not know if the antibody will work in this case. That's the reason our search engine picked this antibody up in the first place.

    Could you please confirm if you want to use this antibody in ICC? Because if you want to use it in IHC-P, this antibody would be indeed suitable for your purpose.

    Read More


    Merci pour votre appel de hier et votre patience.

    J'ai regardé nos anticorps contre l'ubiquitine en un peu plus de détails. La décision n'est pas très facile. Voici mes recommandations/commentaires:

    Le ab33893 est un anticorps monoclonal de lapin. Il donne une très jolie bande sur des cellules Hela. Cet anticorps est relativement populaire et nous avons pas eu de feedback négative depuis plus que deux ans. Je pense que cet anticorps est donc un bon choix. Nous avons pas encore des feedbacks (Abreviews) d'utilisateurs, ceci voudrait dire aussi que vous recevrez plus de points si vous nous donnez des feedbacks. Click here (or use the following: https://www.abcam.com/index.html?datasheet=33893).

    Si vous préférez un anticorps avec plus de feedback (Abreviews) d'autres utilisateurs, je peux vous recommander le ab7780. Ceci est un anticorps très populaire et les utilisateurs semblent être très satisfaits (voir les Abreviews). Des bandes non-spécifiques ont aussi été reporté pour cet anticorps par contre. Comme discuté au téléphone, il est difficile à déterminer si une bande est non-spécifique ou si c'est une protéine ubiquitinylé. Click here (or use the following: https://www.abcam.com/index.html?datasheet=7780).

    J'espère que ces informations vous aideront à faire votre choix. N'hésitez pas à nous recontacter pour plus d'informations.

    Read More


    Thank you for your enquiry. This antibody does indeed cross-react with mouse and rat, in addition to human. I am not sure why the specificity statment was on the datasheet, but I have removed it to avoid future confusion. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

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