Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Ubiquitin antibody [EPR8830]

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
FBS / BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6
Sample
Mouse Tissue sections (moues embryo 20dpc)
Specification
moues embryo 20dpc
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: FBS / BSA 1%
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: LEYCA POWERVISION ANTI RABBIT
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Additional data

Additional Notes
Probably higher dillutions up to 1/500 will also work fine.

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Submitted Sep 09 2013

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