• Product name
    Anti-Ubiquitin antibody [P4G7-H11]
    See all Ubiquitin primary antibodies
  • Description
    Mouse monoclonal [P4G7-H11] to Ubiquitin
  • Host species
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Cow, Human, Schizosaccharomyces pombe
  • Immunogen

    Native bovine Ubiquitin

  • Positive control
    • HeLa, RK-13, 3T3, PC12 and MDBK cell lysates; Mouse and Rat brain tissue extracts
  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.



Our Abpromise guarantee covers the use of ab90376 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    WB: 1/1000. Detects a band of approximately 6.5 kDa (predicted molecular weight: 9 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
    • Cellular localization
      Cell Membrane, Cytoplasmic and Nuclear
    • Database links
    • Alternative names
      • Epididymis secretory protein Li 50 antibody
      • FLJ25987 antibody
      • HEL S 50 antibody
      • MGC8385 antibody
      • Polyubiquitin B antibody
      • RPS 27A antibody
      • RPS27A antibody
      • UBA 52 antibody
      • UBA 80 antibody
      • UBA52 antibody
      • UBA80 antibody
      • UBB antibody
      • UBB_HUMAN antibody
      • UBC antibody
      • UBCEP 1 antibody
      • UBCEP 2 antibody
      • UBCEP1 antibody
      • UBCEP2 antibody
      • Ubiquitin antibody
      • Ubiquitin B antibody
      see all


    • All lanes : Anti-Ubiquitin antibody [P4G7-H11] (ab90376) at 1/1000 dilution

      Lane 1 : Molecular weight marker
      Lane 2 : Ubiquitin
      Lane 3 : HeLa cell lysate
      Lane 4 : RK-13 cell lysate
      Lane 5 : 3T3 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : Mouse brain tissue extract
      Lane 8 : Rat brain tissue extract
      Lane 9 : MDBK cell lysate

      Predicted band size: 9 kDa


    This product has been referenced in:
    • Zhang W  et al. E3 ubiquitin ligase Bre1 couples sister chromatid cohesion establishment to DNA replication inSaccharomyces cerevisiae. Elife 6:N/A (2017). Read more (PubMed: 29058668) »
    • Szilagyi Z  et al. Cyclin-dependent kinase 8 regulates mitotic commitment in fission yeast. Mol Cell Biol 32:2099-109 (2012). WB ; Schizosaccharomyces pombe . Read more (PubMed: 22451489) »

    See all 2 Publications for this product

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