Product nameAnti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (HRP)
See all Ubiquitin primary antibodies
DescriptionRabbit monoclonal [EPR17034] to Ubiquitin (linkage-specific K27) (HRP)
Tested applicationsSuitable for: WBmore details
Predicted to work with: Mouse, Rat, Human
Synthetic peptide within Human Ubiquitin aa 1-100 (linkage-specific K27). The exact sequence is proprietary.
Database link: P0CG48
- WB: Di-Ubiquitin/Ub2 (K27 Linked).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.4
Preservative: 0.1% Proclin
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (ab181537)
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (Alexa Fluor® 488) (ab237254)
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] (Alexa Fluor® 647) (ab237255)
- Anti-Ubiquitin (linkage-specific K27) antibody [EPR17034] - BSA and Azide free (ab238442)
Our Abpromise guarantee covers the use of ab237256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
- Epididymis secretory protein Li 50 antibody
- FLJ25987 antibody
- HEL S 50 antibody
This blot was produced using a 4-12% Bis-Tris gel under the MES buffer system. The gel was run at 200V for 35 mins. before being transferred onto a nitrocellulose membrane at 30V for 70 mins. The membrane was then blocked for an hour using 3% non-fat milk before being incubated with ab237256 (1/1000) overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
Lane 1. Di-Ubiquitin/Ub2 (Full Length) (20ng)
Lane 2. Di-Ubiquitin/Ub2 (K11 Linked) (20ng)
Lane 3. Di-Ubiquitin/Ub2 (K27 Linked) (20ng)
Lane 4. Di-Ubiquitin/Ub2 (K29 Linked) (20ng)
Lane 5. Di-Ubiquitin/Ub2 (K33 Linked ) (20ng)
Lane 6. Di-Ubiquitin/Ub2 (K48 Linked) (20ng)
Lane 7. Di-Ubiquitin/Ub2 (K6 Linked) (20ng)
Lane 8. Di-Ubiquitin/Ub2 (K63 Linked) (20ng)
ab237256 has not yet been referenced specifically in any publications.