RecombinantRabMAb

Recombinant Anti-UBLCP1 antibody [EPR11923] (ab176340)

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Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (ab176340)
  • Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340)
  • Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)
  • Anti-UBLCP1 antibody [EPR11923] (ab176340)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11923] to UBLCP1
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

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Carrier Free

Overview

  • Product name

    Anti-UBLCP1 antibody [EPR11923]
    See all UBLCP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR11923] to UBLCP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, HeLa, and K562 cell lysates. HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab176340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF
1/50 - 1/100.
IP
1/10 - 1/100.
Application notes
Is unsuitable for Flow Cyt or IHC-P.

Target

  • Function

    Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.

  • Tissue specificity

    Broadly expressed, with highest levels in placenta, lung, testis and ovary. Up-regulated in tumor tissues.

  • Sequence similarities

    Contains 1 FCP1 homology domain.
    Contains 1 ubiquitin-like domain.

  • Domain

    The Ubiquitin-like domain mediates interaction with proteasomes.

  • Cellular localization

    Nucleus. Colocalizes with nuclear proteasomes.
  • Information by UniProt

  • Database links

    • Alternative names

      • CPUB1 antibody
      • CTD like phosphatase domain containing protein antibody
      • CTD phosphatase like with ubiquitin domain 1 antibody
      • FLJ25267 antibody
      • MGC10067 antibody
      • Nuclear proteasome inhibitor UBLCP1 antibody
      • UBCP1_HUMAN antibody
      • Ubiquitin like domain containing CTD phosphatase 1 antibody
      • Ubiquitin-like domain-containing CTD phosphatase 1 antibody
      • UBLCP1 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (ab176340)
      Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (ab176340)

      Immunofluorescence analysis of HeLa cells labeling UBLCP1 with ab176340 at 1/50 dilution.

    • Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340)
      Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340)
      All lanes : Anti-UBLCP1 antibody [EPR11923] (ab176340) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 37 kDa

    • Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)
      Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)

      Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab176340 at 1/10 dilution.

    • Anti-UBLCP1 antibody [EPR11923] (ab176340)
      Anti-UBLCP1 antibody [EPR11923] (ab176340)

    References (1)

    Publishing research using ab176340? Please let us know so that we can cite the reference in this datasheet.

    ab176340 has been referenced in 1 publication.

    • Sun S  et al. Phosphatase UBLCP1 controls proteasome assembly. Open Biol 7:N/A (2017). PubMed: 28539385

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