Overview

  • Product name
    Anti-UBLCP1 antibody [EPR11923]
    See all UBLCP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR11923] to UBLCP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBLCP1 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8WVY7

  • Positive control
    • 293T, HeLa, and K562 cell lysates. HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab176340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function
      Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.
    • Tissue specificity
      Broadly expressed, with highest levels in placenta, lung, testis and ovary. Up-regulated in tumor tissues.
    • Sequence similarities
      Contains 1 FCP1 homology domain.
      Contains 1 ubiquitin-like domain.
    • Domain
      The Ubiquitin-like domain mediates interaction with proteasomes.
    • Cellular localization
      Nucleus. Colocalizes with nuclear proteasomes.
    • Information by UniProt
    • Database links
      • Alternative names
        • CPUB1 antibody
        • CTD like phosphatase domain containing protein antibody
        • CTD phosphatase like with ubiquitin domain 1 antibody
        • FLJ25267 antibody
        • MGC10067 antibody
        • Nuclear proteasome inhibitor UBLCP1 antibody
        • UBCP1_HUMAN antibody
        • Ubiquitin like domain containing CTD phosphatase 1 antibody
        • Ubiquitin-like domain-containing CTD phosphatase 1 antibody
        • UBLCP1 antibody
        see all

      Images

      • Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340)
        Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340)
        All lanes : Anti-UBLCP1 antibody [EPR11923] (ab176340) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : HeLa cell lysate
        Lane 3 : K562 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 37 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (ab176340)
        Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (ab176340)

        Immunofluorescence analysis of HeLa cells labeling UBLCP1 with ab176340 at 1/50 dilution.

      • Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)
        Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)

        Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab176340 at 1/10 dilution.

      References

      This product has been referenced in:

      See 1 Publication for this product

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