Recombinant Anti-UBLCP1 antibody [EPR11923] (ab176340)
- Datasheet
- References (1)
- Protocols
Overview
-
Product name
Anti-UBLCP1 antibody [EPR11923]
See all UBLCP1 primary antibodies -
Description
Rabbit monoclonal [EPR11923] to UBLCP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBLCP1 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8WVY7 -
Positive control
- 293T, HeLa, and K562 cell lysates. HeLa cells.
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11923 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab176340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 37 kDa. | |
| ICC/IF | 1/50 - 1/100. | |
| IP | 1/10 - 1/100. |
Target
-
Function
Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome. -
Tissue specificity
Broadly expressed, with highest levels in placenta, lung, testis and ovary. Up-regulated in tumor tissues. -
Sequence similarities
Contains 1 FCP1 homology domain.
Contains 1 ubiquitin-like domain. -
Domain
The Ubiquitin-like domain mediates interaction with proteasomes. -
Cellular localization
Nucleus. Colocalizes with nuclear proteasomes. - Information by UniProt
-
Database links
- Entrez Gene: 134510 Human
- Omim: 609867 Human
- SwissProt: Q8WVY7 Human
- Unigene: 591733 Human
-
Alternative names
- CPUB1 antibody
- CTD like phosphatase domain containing protein antibody
- CTD phosphatase like with ubiquitin domain 1 antibody
see all
Images
-
![Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (ab176340)](https://www.abcam.com/ps/products/176/ab176340/Images/ab176340-203666-ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (ab176340)Immunofluorescence analysis of HeLa cells labeling UBLCP1 with ab176340 at 1/50 dilution.
-
![Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340)](https://www.abcam.com/ps/products/176/ab176340/Images/ab176340-203665-WB.jpg)
Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340)All lanes : Anti-UBLCP1 antibody [EPR11923] (ab176340) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa -
![Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)](https://www.abcam.com/ps/products/176/ab176340/Images/ab176340-203668-IP.jpg)
Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab176340 at 1/10 dilution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Sun S et al. Phosphatase UBLCP1 controls proteasome assembly. Open Biol 7:N/A (2017). Read more (PubMed: 28539385) »
