Anti-UBLCP1 antibody [EPR11923] Recombinant (ab176340)

Product name

Anti-UBLCP1 antibody [EPR11923]
See all UBLCP1 primary antibodies
Description

Rabbit monoclonal [EPR11923] to UBLCP1
Host species

Rabbit
Tested applications

Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBLCP1 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8WVY7
Positive control
- 293T, HeLa, and K562 cell lysates. HeLa cells.
General notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR11923
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab176340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 37 kDa.
ICC/IF		1/50 - 1/100.
IP		1/10 - 1/100.

Application notes

Is unsuitable for Flow Cyt or IHC-P.

Function

Dephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.
Tissue specificity

Broadly expressed, with highest levels in placenta, lung, testis and ovary. Up-regulated in tumor tissues.
Sequence similarities

Contains 1 FCP1 homology domain.
Contains 1 ubiquitin-like domain.
Domain

The Ubiquitin-like domain mediates interaction with proteasomes.
Cellular localization

Nucleus. Colocalizes with nuclear proteasomes.
Target information above from: UniProt accession Q8WVY7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 134510 Human
- Omim: 609867 Human
- SwissProt: Q8WVY7 Human
- Unigene: 591733 Human
Alternative names
- CPUB1 antibody
- CTD like phosphatase domain containing protein antibody
- CTD phosphatase like with ubiquitin domain 1 antibody
- FLJ25267 antibody
- MGC10067 antibody
- Nuclear proteasome inhibitor UBLCP1 antibody
- UBCP1_HUMAN antibody
- Ubiquitin like domain containing CTD phosphatase 1 antibody
- Ubiquitin-like domain-containing CTD phosphatase 1 antibody
- UBLCP1 antibody
see all

Western blot - Anti-UBLCP1 antibody [EPR11923] (ab176340)

All lanes : Anti-UBLCP1 antibody [EPR11923] (ab176340) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 37 kDa
Immunocytochemistry/ Immunofluorescence - Anti-UBLCP1 antibody [EPR11923] (ab176340)

Immunofluorescence analysis of HeLa cells labeling UBLCP1 with ab176340 at 1/50 dilution.
Immunoprecipitation - Anti-UBLCP1 antibody [EPR11923] (ab176340)

Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab176340 at 1/10 dilution.

Sun S et al. Phosphatase UBLCP1 controls proteasome assembly. Open Biol 7:N/A (2017). Read more (PubMed: 28539385) »

Publishing research using ab176340? Please let us know so that we can cite the reference in this datasheet.

Anti-UBLCP1 antibody [EPR11923] (ab176340)