Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11923] to UBLCP1
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Product nameAnti-UBLCP1 antibody [EPR11923]
See all UBLCP1 primary antibodies
DescriptionRabbit monoclonal [EPR11923] to UBLCP1
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human UBLCP1 aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q8WVY7
- 293T, HeLa, and K562 cell lysates. HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab176340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 37 kDa.|
|ICC/IF||1/50 - 1/100.|
|IP||1/10 - 1/100.|
FunctionDephosphorylates 26S nuclear proteasomes, thereby decreasing their proteolytic activity. The dephosphorylation may prevent assembly of the core and regulatory particles (CP and RP) into mature 26S proteasome.
Tissue specificityBroadly expressed, with highest levels in placenta, lung, testis and ovary. Up-regulated in tumor tissues.
Sequence similaritiesContains 1 FCP1 homology domain.
Contains 1 ubiquitin-like domain.
DomainThe Ubiquitin-like domain mediates interaction with proteasomes.
Cellular localizationNucleus. Colocalizes with nuclear proteasomes.
- Information by UniProt
- CPUB1 antibody
- CTD like phosphatase domain containing protein antibody
- CTD phosphatase like with ubiquitin domain 1 antibody
Immunofluorescence analysis of HeLa cells labeling UBLCP1 with ab176340 at 1/50 dilution.
All lanes : Anti-UBLCP1 antibody [EPR11923] (ab176340) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 37 kDa
Western blot analysis on immunoprecipitation pellet from K562 cell lysate using ab176340 at 1/10 dilution.
ab176340 has been referenced in 1 publication.
- Sun S et al. Phosphatase UBLCP1 controls proteasome assembly. Open Biol 7:N/A (2017). PubMed: 28539385