Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, MS, Functional Studies, SDS-PAGE
Product nameUbn-ubiquitinylated substrate
See all Ubiquitin proteins and peptides
Purity> 95 % SDS-PAGE.
High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography.
Expression systemEscherichia coli
Protein lengthProtein fragment
Additional sequence informationSynthesized enzymatically in vitro, produced using wild type ubiquitin. Uniprot ids: P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52). MW ~25-150 kDa
DescriptionRecombinant human Ubiquitin protein
Our Abpromise guarantee covers the use of ab157043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
TECHNICAL NOTE: Under certain conditions these polyubiquitinylated substrates can bind to metal affinity matrices.
USES: Deubiquitinylating enzyme substrates (general/linkage specific), Profiling of DUB linkage-type preference or specificity in combination with other single lysine only polyubiquitinylated substrates (e.g. (K11-only)Ubn-ubiquitinylated substrate), investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 0.02% DTT, 0.605% Tris, 0.88% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Epididymis secretory protein Li 50
- HEL S 50
RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
Cellular localizationCell Membrane, Cytoplasmic and Nuclear
Lane 1 : MW Marker
Lanes 2-3 : Ubiquitin linkage-specific mAb
Lane 2 : Substrate treated with USP2
Lane 3 : Substrate without protease
Lane 1: MW Marker, Lane 2: 2 μg of ab157043, Lane 3: 5 μg of ab157043, Lane 4: 10 μg of ab157043
DUB assay. ab157043 was incubated with USP2. Lane 1: MW Marker, Lane 2: Substrate without protease, Lane 3: Substrate with protease, Lane 4: Protease without substrate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab157043 has not yet been referenced specifically in any publications.