• Nature

  • Source

    Escherichia coli
  • Amino Acid Sequence
    • Species

    • Additional sequence information

      Synthesized enzymatically in vitro, produced using wild type ubiquitin. Uniprot ids: P0CG47 (UBB), P0CG48 (UBC), P62979 (RPS27A), P62987 (UBA52). MW ~25-150 kDa


Our Abpromise guarantee covers the use of ab157043 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    Mass Spectrometry

    Functional Studies


  • Purity

    > 95 % SDS-PAGE.
    High molecular weight conjugates were separated from free ubiquitin by size exclusion chromatography.
  • Form

  • Additional notes

    TECHNICAL NOTE: Under certain conditions these polyubiquitinylated substrates can bind to metal affinity matrices.

    USES: Deubiquitinylating enzyme substrates (general/linkage specific), Profiling of DUB linkage-type preference or specificity in combination with other single lysine only polyubiquitinylated substrates (e.g. (K11-only)Ubn-ubiquitinylated substrate), investigation of polyubiquitin chain recognition by and interaction with ubiquitin binding proteins

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.02% DTT, 0.605% Tris, 0.88% Sodium chloride

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • Epididymis secretory protein Li 50
    • FLJ25987
    • HEL S 50
    • MGC8385
    • Polyubiquitin B
    • RPS 27A
    • RPS27A
    • UBA 52
    • UBA 80
    • UBA52
    • UBA80
    • UBB
    • UBC
    • UBCEP 1
    • UBCEP 2
    • UBCEP1
    • UBCEP2
    • Ubiquitin
    • Ubiquitin B
    see all
  • Relevance

    Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.
  • Cellular localization

    Cell Membrane, Cytoplasmic and Nuclear


  • Lane 1 : MW Marker
    Lanes 2-3 : Ubiquitin linkage-specific mAb

    Lane 2 : Substrate treated with USP2
    Lane 3 : Substrate without protease
  • Lane 1: MW Marker, Lane 2: 2 μg of ab157043, Lane 3: 5 μg of ab157043, Lane 4: 10 μg of ab157043

  • DUB assay. ab157043 was incubated with USP2. Lane 1: MW Marker, Lane 2: Substrate without protease, Lane 3: Substrate with protease, Lane 4: Protease without substrate.


ab157043 has not yet been referenced specifically in any publications.

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