Anti-UBP43/USP18 antibody (ab168478)
Key features and details
- Mouse polyclonal to UBP43/USP18
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-UBP43/USP18 antibody -
Description
Mouse polyclonal to UBP43/USP18 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Orangutan
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Immunogen
Full length protein corresponding to Human UBP43/USP18 aa 1-372.
Sequence:MSKAFGLLRQICQSILAESSQSPADLEEKKEEDSNMKREQPRERPRAWDY PHGLVGLHNIGQTCCLNSLIQVFVMNVDFTRILKRITVPRGADEQRRSVP FQMLLLLEKMQDSRQKAVRPLELAYCLQKCNVPLFVQHDAAQLYLKLWNL IKDQITDVHLVERLQALYTIRVKDSLICVDCAMESSRNSSMLTLPLSLFD VDSKPLKTLEDALHCFFQPRELSSKSKCFCENCGKKTRGKQVLKLTHLPQ TLTIHLMRFSIRNSQTRKICHSLYFPQSLDFSQILPMKRESCDAEEQSGG QYELFAVIAHVGMADSGHYCVYIRNAVDGKWFCFNDSNICLVSWEDIQCT YGNPNYHWQETAYLLVYMKMEC
Database link: NP_059110.2 -
Positive control
- UBP43/USP18 transfected 293T cell line lysate; HeLa cells.
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General notes
This product was previously labelled as UBP43
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168478 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa.
|
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| ICC/IF |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 43 kDa. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Can efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms. -
Sequence similarities
Belongs to the peptidase C19 family. - Information by UniProt
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Database links
- Entrez Gene: 11274 Human
- Entrez Gene: 100171781 Orangutan
- Omim: 607057 Human
- SwissProt: Q9UMW8 Human
- SwissProt: Q5RE63 Orangutan
- Unigene: 38260 Human
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Alternative names
- 43 kDa ISG15 specific protease antibody
- 43 kDa ISG15-specific protease antibody
- EC 3.1.2. antibody
see all
Images
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Western blot - Anti-UBP43/USP18 antibody (ab168478)All lanes : Anti-UBP43/USP18 antibody (ab168478) at 1 µg/ml
Lane 1 : UBP43/USP18 transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 15 µl per lane.
Predicted band size: 43 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-UBP43/USP18 antibody (ab168478)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab168478 has not yet been referenced specifically in any publications.