Key features and details
- Rabbit polyclonal to UBP43/USP18
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-UBP43/USP18 antibody
See all UBP43/USP18 primary antibodies
Species reactivityReacts with: Human
Tested in peptide ELISA.
This product was previously labelled as UBP43
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThis is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.|
FunctionCan efficiently cleave only ISG15 fusions including native ISG15 conjugates linked via isopeptide bonds. Necessary to maintain a critical cellular balance of ISG15-conjugated proteins in both healthy and stressed organisms.
Sequence similaritiesBelongs to the peptidase C19 family.
- Information by UniProt
- 43 kDa ISG15 specific protease antibody
- 43 kDa ISG15-specific protease antibody
- EC 3.1.2. antibody
Western blot using ab4188 at 1/500.
Lane 1: Hela nuclear
Lane 2: Hela
Lane 3: A431
Lane 4: Jurkat
Lane 5: HEK293
Secondary ab: Goat polyclonal to Rabbit IgG H&L (IR680) Pre-Adsorbed (1/5000)
18µg of cell lysate per lane.
Expected molecular weight: 43 kD.
The strong band between the 37kD and 50kD markers is expected to be UBP43/USP18
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab4188 has not yet been referenced specifically in any publications.